The mitogen-activated protein kinase CgHog1 is required for iron homeostasis, adherence and virulence in Candida glabrata

The FEBS Journal
Vivek Kumar SrivastavaRupinder Kaur

Abstract

Candida glabrata has emerged as a major fungal pathogen over the last two decades, although our understanding of its survival strategies inside the mammalian host remains rudimentary. An important requirement for survival in vivo is the ability to acquire critical nutrients such as iron from host niches of varied iron content. In the present study, we demonstrate for the first time that C. glabrata cells respond to high external iron levels via activation of two stress-responsive mitogen-activated protein kinases, CgHog1 and CgSlt2, and lack of either kinase results in sensitivity to the high-iron medium. Furthermore, we show that CgHOG1 deletion led to perturbed iron homeostasis (elevated intracellular iron content and high mitochondrial aconitase activity), reduced survival in macrophages and attenuated virulence in the murine model of disseminated candidiasis. Consistently, several genes implicated in iron acquisition and storage displayed deregulated expression in the Cghog1∆ mutant. Genome-wide transcriptional profiling analysis revealed upregulation of genes implicated in DNA repair, RNA processing and autophagy, and downregulation of genes related to cellular respiration and organonitrogen compound metabolism under iron-...Continue Reading

References

Mar 19, 1993·Science·J L BrewsterM C Gustin
Jul 10, 1999·Clinical Microbiology Reviews·D H Howard
Apr 16, 2002·Molecular Biology of the Cell·María Teresa Rodríguez-ManzanequeEnrique Herrero
Sep 28, 2004·Nature Cell Biology·Xavier EscotéFrancesc Posas
Mar 19, 2005·Science·Renee DomergueBrendan P Cormack
Jul 6, 2005·Current Opinion in Microbiology·Rupinder KaurBrendan P Cormack
Aug 5, 2005·Microbiology·Federico Navarro-GarcíaJesús Pla
Jan 13, 2006·Analytical Biochemistry·Jordi TamaritJoaquim Ros
Apr 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rupinder KaurBrendan P Cormack
Jul 3, 2007·Biochimica Et Biophysica Acta·Raymond E Chen, Jeremy Thorner
Aug 24, 2007·Eukaryotic Cell·Xinhua ZhaoJin-Rong Xu
Dec 12, 2007·Molecular and Cellular Biology·Liangtao LiJerry Kaplan
Jun 13, 2009·Nucleic Acids Research·Yuval Mazor, Martin Kupiec
Jun 23, 2009·Current Opinion in Microbiology·Daniel Kornitzer
Nov 16, 2010·Fungal Genetics and Biology : FG & B·Steffen PriebeAxel A Brakhage
Jan 6, 2011·Molecular Systems Biology·Jennifer J SmithJohn D Aitchison
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Aug 13, 2011·The Journal of Biological Chemistry·Sapan BorahRupinder Kaur
Feb 7, 2012·Biochimica Et Biophysica Acta·Caroline C PhilpottAvery G Frey
Jan 1, 2006·CSH Protocols·Joseph Sambrook, David W Russell
Oct 3, 2012·Genetics·Haruo Saito, Francesc Posas
Dec 21, 2012·Cellular Microbiology·Sascha Brunke, Bernhard Hube
Dec 21, 2012·Science Translational Medicine·Gordon D BrownTheodore C White
Aug 22, 2013·Current Opinion in Microbiology·Caryn E Outten, Angela-Nadia Albetel
Dec 11, 2013·Frontiers in Microbiology·Zeljkica JandricChristoph Schüller

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Citations

Jun 13, 2015·FEMS Yeast Research·Lydia KasperBernhard Hube
Feb 22, 2017·Metallomics : Integrated Biometal Science·Gaurav BairwaJames W Kronstad
Jul 12, 2018·Molecular Microbiology·Telma S MartinsClara Pereira
Nov 5, 2016·Frontiers in Cellular and Infection Microbiology·Pedro PaisMiguel C Teixeira
Aug 17, 2018·Cellular Microbiology·Reinhard BeyerChristoph Schüller
May 6, 2019·Microbiology·Frédéric Devaux, Antonin Thiébaut
Feb 2, 2019·Microorganisms·Kundan KumarRupinder Kaur
Jul 15, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·María Teresa Martínez-Pastor, Sergi Puig

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