Abstract
Protein kinase B (Akt) and the mitogen-activated protein kinases (MAPKs) mediate hypertrophy in the adult heart, although their importance in the developing heart is poorly understood. The goal of the current study was to determine if volume loading the fetal heart resulting from chronic anemia affects regulation of Akt and the MAPKs and if this response is developmentally regulated. Anemia was created by 7 days of isovolumic hemorrhage beginning at 101 days (early GA) or 129 days (late GA) gestational age (GA) in fetal sheep (term = 145 days), following which protein levels of total and active Akt, extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun NH2-terminal kinase (JNK), and p38 were determined in the right and left ventricle (RV and LV). RV protein-to-DNA ratios were also assessed. At both GAs, ventricular (RV + LV + septum) weight normalized to body weight was significantly increased in anemic fetuses. Anemia had no effect on expression of myocardial total or active Akt, JNK, or p38 at either GA. Levels of total ERK1/2 were also unchanged, although active ERK1/2 was significantly decreased in the late but not early GA anemic fetuses. Total JNK and total and active ERK1/2 and Akt were significantly greater in early...Continue Reading
References
Apr 29, 1972·The Veterinary Record·J J Jennings, J P Crowley
Nov 1, 1984·Hypertension·S OparilF J Clubb
Mar 15, 1996·The Journal of Biological Chemistry·Y ChuK Kelly
Feb 21, 1997·The Journal of Biological Chemistry·M MudaS Arkinstall
Apr 16, 1998·American Journal of Obstetrics and Gynecology·C MartinG L Semenza
Mar 14, 2000·Current Opinion in Cell Biology·S M Keyse
Mar 23, 2000·Cardiovascular Research·M A Bogoyevitch
Sep 27, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·A BarberaK L Thornburg
Jan 5, 2001·Circulation Research·O F BuenoJ D Molkentin
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·P LiaoY Wang
Nov 14, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·J L SegarT D Scholz
Nov 21, 2001·The Journal of Clinical Investigation·H YasukawaK R Chien
Feb 22, 2002·European Journal of Biochemistry·Shinri TamuraTakayasu Kobayashi
Apr 27, 2002·Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·Shinri TamuraTakayasu Kobayashi
Nov 2, 2002·Circulation Research·Orlando F Bueno, Jeffery D Molkentin
Mar 11, 2003·The Journal of Physiology·N C SundgrenK L Thornburg
Aug 30, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Nathan C SundgrenKent L Thornburg
Sep 16, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Judith H BurrellEugenie R Lumbers
Oct 1, 2003·The EMBO Journal·Qiangrong LiangJeffery D Molkentin
Dec 5, 2003·Journal of Molecular and Cellular Cardiology·Qiangrong Liang, Jeffery D Molkentin
Jul 28, 2004·Journal of Molecular and Cellular Cardiology·Gavin Y OuditPeter H Backx
May 5, 2005·Genes & Development·Felix B EngelMark T Keating
Citations
Feb 7, 2009·Tissue Engineering. Part a·Kelly C ClauseKimimasa Tobita
Jul 11, 2012·Clinical and Experimental Pharmacology & Physiology·Kimberley C W WangJanna L Morrison
Mar 10, 2011·Pediatric Research·Sonnet S JonkerJeffrey L Segar
Mar 13, 2014·Pediatric Research·Andrew W NorrisJeffrey L Segar
Feb 18, 2011·Canadian Journal of Physiology and Pharmacology·Sonnet S JonkerJeffrey L Segar
Aug 3, 2011·The Journal of Physiology·Kimberley C W WangJanna L Morrison
Aug 25, 2009·Experimental Physiology·Sonnet S JonkerKent L Thornburg
Dec 7, 2007·Aesthetic Plastic Surgery·B S Atiyeh, S N Hayek
Jun 4, 2010·The Journal of Physiology·P F O'TierneyK L Thornburg
Oct 30, 2012·Experimental Physiology·Jeffrey L SegarThomas D Scholz
Sep 20, 2007·Neonatology·Aaron K OlsonJeffrey L Segar
Mar 27, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jeremy SandgrenJeffrey L Segar