The Mitotic and Metabolic Effects of Phosphatidic Acid in the Primary Muscle Cells of Turbot (Scophthalmus maximus )

Frontiers in Endocrinology
Tingting WangGen He

Abstract

Searching for nutraceuticals and understanding the underlying mechanism that promote fish growth is at high demand for aquaculture industry. In this study, the modulatory effects of soy phosphatidic acids (PA) on cell proliferation, nutrient sensing, and metabolic pathways were systematically examined in primary muscle cells of turbot (Scophthalmus maximus). PA was found to stimulate cell proliferation and promote G1/S phase transition through activation of target of rapamycin signaling pathway. The expression of myogenic regulatory factors, including myoD and follistatin, was upregulated, while that of myogenin and myostatin was downregulated by PA. Furthermore, PA increased intracellular free amino acid levels and enhanced protein synthesis, lipogenesis, and glycolysis, while suppressed amino acid degradation and lipolysis. PA also was found to increased cellular energy production through stimulated tricarboxylic acid cycle and oxidative phosphorylation. Our results identified PA as a potential nutraceutical that stimulates muscle cell proliferation and anabolism in fish.

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Citations

Sep 12, 2020·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Kim H H LissBrian N Finck
Feb 5, 2021·International Journal of Molecular Sciences·Ana Gomez-LarrauriAlberto Ouro
Jun 27, 2019·Journal of Agricultural and Food Chemistry·Naoko Goto-InoueTsukasa Mori

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Methods Mentioned

BETA
Assay
Flow
flow cytometry
Protein Assay
PCR
electrophoresis

Software Mentioned

NIH Image
SPSS

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