The mitotic arrest deficient protein MAD2B interacts with the small GTPase RAN throughout the cell cycle

PloS One
Klaas MedendorpAd Geurts van Kessel


Previously, we identified the mitotic arrest deficient protein MAD2B (MAD2L2) as a bona fide interactor of the renal cell carcinoma (RCC)-associated protein PRCC. In addition, we found that fusion of PRCC with the transcription factor TFE3 in t(X;1)(p11;q21)-positive RCCs results in an impairment of this interaction and, concomitantly, an abrogation of cell cycle progression. Although MAD2B is thought to inhibit the anaphase promoting complex (APC) by binding to CDC20 and/or CDH1(FZR1), its exact role in cell cycle control still remains to be established. Using a yeast two-hybrid interaction trap we identified the small GTPase RAN, a well-known cell cycle regulator, as a novel MAD2B binding protein. Endogenous interaction was established in mammalian cells via co-localization and co-immunoprecipitation of the respective proteins. The interaction domain of RAN could be assigned to a C-terminal moiety of 60 amino acids, whereas MAD2B had to be present in its full-length conformation. The MAD2B-RAN interaction was found to persist throughout the cell cycle. During mitosis, co-localization at the spindle was observed. The small GTPase RAN is a novel MAD2B binding protein. This novel protein-protein interaction may play a role in (i...Continue Reading


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Oct 9, 2013·The Journal of Cell Biology·Tamar Listovsky, Julian E Sale
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Nov 25, 2015·The Journal of Cell Biology·Scott C Rosenberg, Kevin D Corbett
Jan 12, 2013·Journal of Proteomics·José L Martínez-SalgadoAna P Barba de la Rosa
Aug 17, 2018·Proceedings of the National Academy of Sciences of the United States of America·Alessandro A RizzoDmitry M Korzhnev
Aug 30, 2018·The EMBO Journal·Steven FindlayAlexandre Orthwein
Apr 20, 2020·Environmental and Molecular Mutagenesis·Kylie FackrellJunya Tomida
Apr 26, 2020·Nature Communications·Ling LiangYuxin Yin
Aug 15, 2019·Nucleic Acids Research·Sara K Martin, Richard D Wood


Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·L T da CostaB Vogelstein
Oct 11, 1996·Science·Y Li, R Benezra
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·C GieffersJ M Peters
Sep 7, 2000·Cell·M A Hoyt
Feb 13, 2001·Cell·J V Shah, D W Cleveland
Jul 19, 2001·Genes & Development·J Chen, G Fang
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·M A WetermanAd Geurts van Kessel
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Andrea Musacchio, Kevin G Hardwick
Dec 11, 2002·Current Opinion in Cell Biology·Hongtao Yu
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Yixian Zheng
Feb 23, 2005·Current Biology : CB·Kevin G Hardwick
Mar 31, 2005·Cell·Kim Nasmyth
May 26, 2005·Investigative Ophthalmology & Visual Science·Albena KantardzhievaJan Wijnholds
Dec 16, 2005·Nature·Karthik B JeganathanJan M van Deursen
Feb 17, 2006·Genes & Development·Brian R ThorntonDavid P Toczyski
Mar 31, 2006·Nature·Petr KalábKarsten Weis
Jun 7, 2006·Current Biology : CB·Péter Lénárt, Jan-Michael Peters
Jun 20, 2006·Current Biology : CB·Helen S Sanderson, Paul R Clarke
May 8, 2007·Cellular and Molecular Life Sciences : CMLS·M CiciarelloP Lavia
Jun 2, 2007·Molecular and Cellular Biochemistry·Li LiKeke Huo
May 16, 2009·The Journal of Biological Chemistry·Chun-Fu HongCheng-Wen Wu

Related Concepts

Pathologic Cytolysis
Cell Cycle Progression
Establishment and Maintenance of Localization
Yeast Two-Hybrid System Techniques
Benign Neoplasm of Testis
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