PMID: 8585957Jan 1, 1996Paper

The mixed lineage leukemia (MLL) protein involved in 11q23 translocations contains a domain that binds cruciform DNA and scaffold attachment region (SAR) DNA

Current Topics in Microbiology and Immunology
P L BroekerN Zeleznik-Le

Abstract

Translocations involving chromosome band 11q23, found in acute lymphoid and myeloid leukemias, disrupt the MLL gene. This gene encodes a putative transcription factor with regions of homology to several other proteins including the zinc fingers and other domains of the Drosophila trithorax gene product, and the "AT-hook" DNA-binding motif of high mobility group proteins. We have previously demonstrated that MLL contains transcriptional activation and repression domains using a GAL4 fusion protein system (21). The repression domain, which is capable of repressing transcription 3-5-fold, is located centromeric to the breakpoint region of MLL. The activation domain, located telomeric to the breakpoint region, activated transcription from a variety of promoters including ones containing only basal promoter elements. The level of activation was very high, ranging from 10-fold to more than 300-fold, depending on the promoter and cell line used for transient transfection. In translocations involving MLL, the protein produced from the der(11) chromosome which contains the critical junction for leukemogenesis includes the AT-hook domain and the repression domain. We assessed the DNA binding capability of the MLL AT-hook domain using bac...Continue Reading

Citations

May 29, 2002·Oncogene·Joseph M ScanduraStephen D Nimer
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·C CasliniJ L Hess
Jun 21, 2012·British Journal of Haematology·Bastian Scholz, Rolf Marschalek
Sep 6, 2001·Genesis : the Journal of Genetics and Development·P AytonV Subramanian

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