The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis

Molecular Cell
Markus TeigeHeribert Hirt

Abstract

The Arabidopsis mitogen-activated protein kinase (MAPK) kinase 2 (MKK2) and the downstream MAPKs MPK4 and MPK6 were isolated by functional complementation of osmosensitive yeast mutants. In Arabidopsis protoplasts, MKK2 was specifically activated by cold and salt stress and by the stress-induced MAPK kinase kinase MEKK1. Yeast two-hybrid, in vitro, and in vivo protein kinase assays revealed that MKK2 directly targets MPK4 and MPK6. Accordingly, plants overexpressing MKK2 exhibited constitutive MPK4 and MPK6 activity, constitutively upregulated expression of stress-induced marker genes, and increased freezing and salt tolerance. In contrast, mkk2 null plants were impaired in MPK4 and MPK6 activation and were hypersensitive to salt and cold stress. Full genome transcriptome analysis of MKK2-overexpressing plants demonstrated altered expression of 152 genes involved in transcriptional regulation, signal transduction, cellular defense, and stress metabolism. These data identify a MAP kinase signaling cascade mediating cold and salt stress tolerance in plants.

References

May 1, 1992·The Plant Journal : for Cell and Molecular Biology·M MinetF Lacroute
Jul 24, 1987·Nucleic Acids Research·R TöpferH H Steinbiss
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·M C GustinK Davenport
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Jan 8, 1999·Biochemical and Biophysical Research Communications·K IchimuraK Shinozaki
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Mar 14, 2000·The Journal of Biological Chemistry·T S NühseT Boller
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y KovtunJ Sheen
Sep 7, 2000·The Journal of Biological Chemistry·F CardinaleH Hirt
Dec 15, 2000·Plant Physiology·T Zhu, X Wang
Dec 21, 2000·The Plant Journal : for Cell and Molecular Biology·K IchimuraK Shinozaki
Dec 29, 2000·Nature·UNKNOWN Arabidopsis Genome Initiative
Feb 24, 2001·Proceedings of the National Academy of Sciences of the United States of America·K Y YangS Zhang
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·M TeigeG Ammerer
Oct 13, 2001·Current Opinion in Plant Biology·G TenaJ Sheen
Oct 13, 2001·Current Opinion in Plant Biology·J K Zhu
Nov 10, 2001·Trends in Plant Science·S Zhang, D F Klessig
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Stefan Hohmann
Jun 5, 2002·The Plant Cell·Liming XiongJian-Kang Zhu
Jul 18, 2002·Trends in Plant Science·UNKNOWN MAPK Group
Jul 27, 2002·Trends in Genetics : TIG·Sean M O'RourkeErin K O'Shea
Aug 17, 2002·Current Opinion in Plant Biology·Claudia JonakHeribert Hirt
Dec 14, 2002·Plant Physiology·Joel A KrepsJeff F Harper
Mar 1, 2003·The Plant Journal : for Cell and Molecular Biology·Hailing JinShuqun Zhang
Mar 12, 2003·The EMBO Journal·Fatma OuakedHeribert Hirt
Mar 21, 2003·The Journal of Biological Chemistry·Irute MeskieneHeribert Hirt

❮ Previous
Next ❯

Citations

Apr 2, 2011·Plant Cell Reports·Hyeong Cheol ParkWoo Sik Chung
Dec 6, 2005·Functional & Integrative Genomics·Kanako KawauraYasunari Ogihara
Jul 17, 2013·Journal of Plant Research·Tomoyuki FuruyaTakashi Nanmori
Apr 18, 2007·Plant Molecular Biology·Antonio F MonroyPatrick J Gulick
Mar 6, 2010·Plant Molecular Biology·Ikram ZaïdiMoez Hanin
Jun 25, 2013·Plant Molecular Biology·Elisa Ferro, Lorenza Trabalzini
Apr 28, 2012·Molecular Biotechnology·Pradeep K AgarwalBhavanath Jha
Jun 15, 2005·Trends in Plant Science·Hirofumi NakagamiHeribert Hirt
Nov 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Fabien JammesJune M Kwak
Dec 23, 2009·The Journal of Biological Chemistry·Tianbao YangB W Poovaiah
Jan 1, 2010·Rejuvenation Research·Meytal DamriGideon Grafi
Feb 1, 2012·Journal of Experimental Botany·Julia Krasensky, Claudia Jonak
Apr 3, 2012·Journal of Experimental Botany·Nicky J Atkinson, Peter E Urwin
Aug 11, 2011·Plant & Cell Physiology·Artur CondeHernâni Gerós
May 18, 2007·Molecular Plant-microbe Interactions : MPMI·Günter BraderHeribert Hirt
Dec 20, 2008·Genes & Development·Sorina C PopescuSavithramma P Dinesh-Kumar
Jul 13, 2005·Plant Physiology·Marie Boudsocq, Christiane Laurière
May 2, 2006·Plant Physiology·Willem Van de VeldeSofie Goormachtig
Jun 9, 2006·Plant Physiology·Andrea Pitzschke, Heribert Hirt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.