The modal distribution of protein isoelectric points reflects amino acid properties rather than sequence evolution

Proteomics
Georg F WeillerNicole Sylvester

Abstract

Two-dimensional gel electrophoresis, a routine application in proteomics, separates proteins according to their molecular mass (M(r)) and isoelectric point (pI). As the genomic sequences for more and more organisms are determined, the M(r) and pI of all their proteins can be estimated computationally. The examination of several of these theoretical proteome plots has revealed a multimodal pI distribution, however, no conclusive explanation for this unusual distribution has so far been presented. We examined the pI distribution of 115 fully sequenced genomes and observed that the modal distribution does not reflect phylogeny or sequence evolution, but rather the chemical properties of amino acids. We provide a statistical explanation of why the observed distributions of pI values are multimodal.

References

Aug 17, 2012·Journal of the American Society for Mass Spectrometry·Jared B ShawJennifer S Brodbelt
Oct 24, 2009·BMC Biology·Pedro Chan, Jim Warwicker
Feb 23, 2008·Bioinformation·Oliviero Carugo
Oct 2, 2014·BMC Genomics·Hanwei YanBeijiu Cheng
Jun 12, 2009·Electrophoresis·Elisabetta Gianazza, Pier Giorgio Righetti
Dec 1, 2005·Proteomics·Songfeng WuFuchu He
May 18, 2006·Proteomics·Pedro ChanJim Warwicker
Sep 6, 2012·MicrobiologyOpen·Elke LecoutereRob Lavigne
Jun 8, 2012·Proteins·Mark A SeegerSarah E Rice
Aug 30, 2005·Trends in Biochemical Sciences·Jan J Spitzer, Bert Poolman
Feb 22, 2012·Protein Expression and Purification·Bruno CoutardChristophe Bignon
Dec 3, 2014·Journal of Molecular Biology·Stephen S MacKinnon, Shoshana J Wodak
Oct 23, 2016·Biology Direct·Lukasz P Kozlowski
Mar 6, 2012·Biochimica Et Biophysica Acta·Rouslan G Efremov, Leonid A Sazanov
Dec 9, 2017·Journal of Molecular Recognition : JMR·Meryl WangBuyong Ma
Nov 3, 2018·Proceedings of the National Academy of Sciences of the United States of America·Kaiser Loell, Vikas Nanda
Jun 4, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Zandra E WaltonChi V Dang
Aug 21, 2019·BMC Molecular and Cell Biology·Atsushi KurotaniTetsuya Sakurai
Aug 7, 2019·BMC Genomics·Tapan Kumar MohantaAhmed Al-Harrasi
Aug 24, 2019·Microbiome·Pedro J Cabello-Yeves, Francisco Rodriguez-Valera
Dec 6, 2006·The Journal of Cell Biology·Davide MarenduzzoPeter R Cook

Related Concepts

Genome
Amino Acids, I.V. solution additive
Proteomics
Genomics
Proteome
2D Polyacrylamide Gel Electrophoresis
Amino Acid [EPC]
Electrophoresis, Gel, Two-Dimensional
Computational Molecular Biology
Antifibrinolytic amino acids

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Christopher G KnightPaul B Rainey
Proceedings of the National Academy of Sciences of the United States of America
T KawashimaM Suzuki
© 2020 Meta ULC. All rights reserved