The mode of binding ACMA-DNA relies on the base-pair nature

Organic & Biomolecular Chemistry
Natalia BustoMarcella Venturini

Abstract

A thermodynamic and kinetic study on the mode of binding of 9-amino-6-chloro-2-methoxi-acridine (ACMA) to poly(dA-dT)·poly(dA-dT) and poly(dG-dC)·poly(dG-dC) has been undertaken at pH = 7.0 and I = 0.1 M. The spectrophotometric, kinetic (T-jump), circular dichroism, viscometric and calorimetric information gathered point to formation of a fully intercalated ACMA complex with poly(dA-dT)·poly(dA-dT) and another one only partially intercalated (7%) with poly(dG-dC)·poly(dG-dC). The ACMA affinity with the A-T bases was higher than with the G-C bases. The two polynucleotide sequences give rise to external complexes when the ACMA concentration is raised, namely, the electrostatic complex poly(dA-dT)·poly(dA-dT)-ACMA and the major groove binding complex poly(dG-dC)·poly(dG-dC)-ACMA. A considerable quenching effect of the ACMA fluorescence is observed with poly(dA-dT)·poly(dA-dT), ascribable to face-to-face location in the intercalated A-T-ACMA base-pairs. The even stronger effect observed in the presence of poly(dG-dC)·poly(dG-dC) is related to the guanine residue from on- and off-slot ACMA positions.

References

Jul 1, 1975·Analytica Chimica Acta·A C Capomacchia, S G Schulman
Mar 19, 1970·Biochimica Et Biophysica Acta·L M Chan, J A McCarter
Sep 20, 2001·Current Pharmaceutical Design·M F BrañaA Ramos
Dec 6, 2002·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Stephan HessJ Luis Pérez Lustres
Dec 17, 2004·Journal of the American Chemical Society·Anna TikhomirovaTigran V Chalikian
Jun 16, 2006·Journal of Bioenergetics and Biomembranes·Richard E McCarty
May 15, 2007·Mutation Research·Lynnette R Ferguson, William A Denny
Jun 8, 2010·The Journal of Physical Chemistry. B·Begoña GarcíaM Venturini
Oct 6, 2011·Physical Chemistry Chemical Physics : PCCP·Natalia BustoFernando Secco

❮ Previous
Next ❯

Citations

Feb 8, 2018·Chemical Biology & Drug Design·Vanessa de Lima SerafimRicardo Olímpio de Moura
Nov 12, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Rubén Gil-GarcíaJavier García-Tojal
May 15, 2015·Angewandte Chemie·David BucetaM Arturo López-Quintela
Dec 12, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Jesús ValladolidBegoña García
Jun 11, 2018·Journal of Colloid and Interface Science·Elia GruesoF Muriel-Delgado
Jan 15, 2014·The Journal of Physical Chemistry. B·Cristina Pérez-ArnaizBegoña García

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.