The modes of action of gallamine

Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character
D Colquhoun, R E Sheridan

Abstract

The action of gallamine, a classical competitive neuromuscular blocking agent, has been examined on voltage-clamped endplates of frog skeletal muscle fibres. Gallamine produces a parallel shift of the equilibrium log (concentration)--response curves in concentrations of up to about 40 microM. At a membrane potential of -70 mV the Schild plot of the dose ratios so measured has a gradient of slightly less than the theoretical value, for a competitive antagonist, of unity. The apparent equilibrium constant for 'competitive' block is about 2 microM, and is approximately independent of the membrane potential. Fluctuation analysis of the endplate current shows two components in the presence of gallamine. The results can be fitted, over the range tested, by a mechanism that involves block of open ion channels by gallamine in a manner similar to that by procaine or quaternary local anaesthetic analogues. The rate constants for this action are strongly dependent on the membrane potential. At -100 mV the association rate constant is about 4 x 10(7) M-1S-1, the dissociation rate constant is about 600 s-1, and the equilibrium constant about 15 microM. Other kinetic measurements (voltage-jump relaxation, and nerve-evoked endplate currents) ...Continue Reading

References

Apr 1, 1978·The Journal of Physiology·E Neher, J H Steinbach
Aug 1, 1979·The Journal of Physiology·D ColquhounR E Sheridan
Nov 1, 1976·British Journal of Pharmacology·A L Clark, F Mitchelson
Dec 1, 1971·The Biochemical Journal·J A SturmanG E Gaull
Nov 1, 1970·Canadian Anaesthetists' Society Journal·F J Rathbun, J T Hamilton
Mar 6, 1981·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D Colquhoun, A G Hawkes
Apr 1, 1958·The American Journal of Physiology·K KOKETSU
Apr 1, 1965·British Journal of Pharmacology and Chemotherapy·J J JONES, J L LAITY
Oct 1, 1951·Annals of the New York Academy of Sciences·W F RIKER, W C WESCOE

❮ Previous
Next ❯

Citations

Aug 21, 1980·The Journal of Membrane Biology·D Marchais, A Marty
Jan 1, 1988·Pharmacology & Therapeutics·F J Barrantes
Nov 1, 1986·Journal of Neuroscience Methods·J Dempster
Jun 1, 1986·The Journal of General Physiology·J A SanchezB Hille
May 1, 1987·Clinical and Experimental Pharmacology & Physiology·F J Mitchelson
Jan 1, 1991·British Journal of Pharmacology·D J AdamsD A Terrar
Jun 6, 2012·Neuroscience Research·Saki TanimotoHiromu Yawo
Dec 1, 1983·Cell Calcium·D H JenkinsonN S Cook
Mar 1, 1984·British Journal of Pharmacology·H P Rang, R J Rylett
Jun 22, 1984·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P T GrayJ M Ritchie
Sep 23, 1985·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D C Ogden, D Colquhoun
Jul 1, 1991·Journal of Bioluminescence and Chemiluminescence·Y de Rautlin de la RoyB Grignon
Mar 6, 1981·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D Colquhoun, A G Hawkes
Jun 6, 2003·Perception & Psychophysics·Martin Arguin, E Charles Leek

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.