PMID: 9643644Jun 27, 1998Paper

The modification of alveolar bone proteoglycans by reactive oxygen species in vitro

Connective Tissue Research
R MoseleyS G Rees

Abstract

Reactive oxygen species (ROS) are being increasingly implicated in the connective tissue degradation associated with chronic inflammatory conditions, such as periodontal disease. The present study investigated the effects of ROS on the proteoglycans (PG) of alveolar bone which are important structural components within the periodontium. PG were isolated from ovine alveolar bone and exposed to increasing concentrations of hydrogen peroxide (H2O2) or to a hydroxyl radical (.OH) flux for 1 h or 24 h, and the degradation products examined for depolymerisation and chemical modification of the PG structure. ROS were demonstrated to be capable of degrading alveolar bone PG in vitro, the .OH species resulting in greater modification than H2O2. The degradative effects observed included cleavage of the protein core and depolymerisation of the GAG chains. The core proteins were more susceptible to degradation than the GAG chains in the presence of H2O2 alone, although both the core proteins and the GAG chains were extensively degraded in the presence of a .OH flux for both 1 h and 24 h. Exposure of the PG to .OH for 24 h resulted in significant modification to the amino acid composition with decreases in the proportion of leucine and the ...Continue Reading

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Citations

May 14, 2014·Cell Biochemistry and Biophysics·Hasan Aksit, Aysegül Bildik
Apr 22, 2008·Free Radical Biology & Medicine·Martin D ReesMichael J Davies
Jan 12, 2007·Periodontology 2000·Iain L C Chapple, John B Matthews
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Apr 2, 2002·Biochemical and Biophysical Research Communications·Sarah G ReesGraham Embery
Dec 26, 2001·Biochimica Et Biophysica Acta·S G ReesG Embery

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