The modification of Gat1p in nitrogen catabolite repression to enhance non-preferred nitrogen utilization in Saccharomyces cerevisiae

Scientific Reports
Xinrui ZhaoJingwen Zhou

Abstract

In Saccharomyces cerevisiae, when preferred nitrogen sources are present, the metabolism of non-preferred nitrogen is repressed. Previous work showed that this metabolic regulation is primarily controlled by nitrogen catabolite repression (NCR) related regulators. Among these regulators, two positive regulators (Gln3p and Gat1p) could be phosphorylated and sequestered in the cytoplasm leading to the transcription of non-preferred nitrogen metabolic genes being repressed. The nuclear localization signals (NLSs) and nuclear localization regulatory signals (NLRSs) in Gln3p and Gat1p play essential roles in the regulation of their localization in cells. However, compared with Gln3p, the information of NLS and NLRS for Gat1p remains unknown. In this study, residues 348-375 and 366-510 were identified as the NLS and NLRS of Gat1p firstly. In addition, the modifications of Gat1p (mutations on the NLS and truncation on the NLRS) were attempted to enhance the transcription of non-preferred nitrogen metabolic genes. Quantitative real-time PCR showed that the transcriptional levels of 15 non-preferred nitrogen metabolic genes increased. Furthermore, during the shaking-flask culture tests, the utilization of urea, proline and allantoine wa...Continue Reading

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Citations

Feb 8, 2018·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Weiping ZhangJian Chen
Jul 10, 2019·World Journal of Microbiology & Biotechnology·Peng ZhangXing Hu
Aug 13, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tianyu WeiQihe Chen
May 11, 2021·World Journal of Microbiology & Biotechnology·Xinchao YangMaihai Guo
Jul 30, 2021·Microbiological Research·Abhinav Nair, Saurabh Jyoti Sarma

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Methods Mentioned

BETA
fluorescence microscopy

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