The modified wobble nucleoside uridine-5-oxyacetic acid in tRNAPro(cmo5UGG) promotes reading of all four proline codons in vivo

RNA
S Joakim NasvallGlenn R Bjork

Abstract

In Salmonella enterica serovar Typhimurium five of the eight family codon boxes are decoded by a tRNA having the modified nucleoside uridine-5-oxyacetic acid (cmo5U) as a wobble nucleoside present in position 34 of the tRNA. In the proline family codon box, one (tRNAProcmo5UGG) of the three tRNAs that reads the four proline codons has cmo5U34. According to theoretical predictions and several results obtained in vitro, cmo5U34 should base pair with A, G, and U in the third position of the codon but not with C. To analyze the function of cmo5U34 in tRNAProcmo5UGG in vivo, we first identified two genes (cmoA and cmoB) involved in the synthesis of cmo5U34. The null mutation cmoB2 results in tRNA having 5-hydroxyuridine (ho5U34) instead of cmo5U34, whereas the null mutation cmoA1 results in the accumulation of 5-methoxyuridine (mo5U34) and ho5U34 in tRNA. The results suggest that the synthesis of cmo5U34 occurs as follows: U34 -->(?) ho5U -->(CmoB) mo5U -->(CmoA?) cmo5U. We introduced the cmoA1 or the cmoB2 null mutations into a strain that only had tRNAProcmo5UGG and thus lacked the other two proline-specific tRNAs normally present in the cell. From analysis of growth rates of various strains and of the frequency of +1 frameshiftin...Continue Reading

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·U Lagerkvist
Mar 1, 1978·Nucleic Acids Research·W T PopeR H Reeves
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S YokoyamaT Miyazawa
Jan 1, 1972·Molecular & General Genetics : MGG·H Schmieger
Jun 7, 1974·Nature·J F Atkins, S Ryce
Aug 1, 1966·Journal of Molecular Biology·F H Crick
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jan 10, 2002·Biochemical and Biophysical Research Communications·Kevin L Griffith, Richard E Wolf
Jan 13, 2004·Nucleic Acids Research·Paul F Agris
Apr 15, 2004·Journal of Molecular Biology·Steven S PhelpsSimpson Joseph

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Citations

Apr 5, 2014·Journal of Chemical Information and Modeling·Indrajit DebAnsuman Lahiri
Aug 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Tatsuya MaehigashiChristine M Dunham
May 15, 2007·Nature Structural & Molecular Biology·Albert WeixlbaumerV Ramakrishnan
Mar 12, 2008·Molecular and Cellular Biology·Marcus J O JohanssonAnders S Byström
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·John F Atkins, Glenn R Björk
Mar 24, 2009·Nature Structural & Molecular Biology·Sarah LedouxOlke C Uhlenbeck
Apr 27, 2013·BMC Structural Biology·Jungwook Kim, Steven C Almo
Feb 3, 2011·Nature Communications·Erwin van den BornPål Ø Falnes
Oct 2, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Ronald C TaylorH Steven Wiley
Jul 4, 2015·International Journal of Molecular Sciences·Howard B GamperYa-Ming Hou
Jul 20, 2010·Current Opinion in Biotechnology·Michael C Jewett, Anthony C Forster
Dec 26, 2015·Nucleic Acids Research·Nandini ManickamPhilip J Farabaugh
Nov 18, 2008·Journal of Molecular Biology·S Joakim NäsvallGlenn R Björk
Apr 2, 2008·Current Opinion in Microbiology·Estella M GustiloPaul F Agris
Sep 9, 2015·Journal of Molecular Biology·M J LajoieG M Church
Dec 26, 2006·Journal of Molecular Biology·Paul F AgrisWilliam D Graham
Aug 19, 2015·Nucleic Acids Research·Hasan TükenmezAnders S Byström
Jan 15, 2008·Nature Structural & Molecular Biology·Marcelo RogalskiRalph Bock
Nov 2, 2005·Trends in Biochemical Sciences·Taraka Dale, Olke C Uhlenbeck
May 27, 2015·Nature Communications·Howard B GamperYa-Ming Hou
Aug 28, 2012·Trends in Genetics : TIG·Eva Maria Novoa, Lluís Ribas de Pouplana
Jan 16, 2007·Molecular Cell·Ute Kothe, Marina V Rodnina
Jun 7, 2014·Frontiers in Genetics·Hiroyuki Hori
Jun 30, 2010·PLoS Genetics·Fran SupekTomislav Smuc
Jul 23, 2013·PLoS Genetics·Jorge Fernández-VázquezElena Hidalgo
Jul 11, 2008·Molecular Cell·Sarah Ledoux, Olke C Uhlenbeck
Oct 21, 2005·Journal of Molecular Biology·Michael A SørensenMåns Ehrenberg
May 23, 2013·Acta Crystallographica. Section D, Biological Crystallography·Robert T ByrneDavid G Waterman
Apr 5, 2017·Biomolecules·Christian LorenzMario Mörl
Oct 27, 2017·Nucleic Acids Research·Satoshi KimuraTsutomu Suzuki

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