DOI: 10.1101/481820Nov 28, 2018Paper

The modular mechanism of chromocenter formation in Drosophila

BioRxiv : the Preprint Server for Biology
Madhav JagannathanYukiko M Yamashita

Abstract

A central principle underlying the ubiquity and abundance of pericentromeric satellite DNA repeats in eukaryotes has remained poorly understood. In our previous study (Jagannathan et al., 2018), we proposed that the interchromosomal clustering of satellite DNAs into nuclear structures known as chromocenters ensures encapsulation of all chromosomes into a single nucleus. Chromocenter disruption led to micronuclei formation, resulting in cell death. Here we show that chromocenter formation is mediated by a ‘modular’ network, where interactions between two sequence-specific satellite DNA-binding proteins, D1 and Prod, bound to their cognate satellite DNAs, bring the full complement of chromosomes into the chromocenter. D1 prod double mutants die during embryogenesis, exhibiting enhanced phenotypes associated with chromocenter disruption, revealing the universal importance of satellite DNAs and chromocenters. Taken together, we propose that interactions between chromocenter modules, consisting of satellite DNA binding proteins and their cognate satellite DNA, package the Drosophila genome within a single nucleus.

Related Concepts

Cell Death
Cell Nucleus
Chromosomes
DNA
DNA, Satellite
DNA-Binding Proteins
Drosophila
Embryonic Development
Genome
HSP40 Heat-Shock Proteins

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