The modulation of acetic acid pathway genes in Arabidopsis improves survival under drought stress

Scientific Reports
Sultana RasheedMotoaki Seki

Abstract

The Arabidopsis histone deacetylase 6 (HDA6) mutant exhibits increased tolerance to drought stress by negatively regulating the expression of ALDH2B7 and PDC1. Therefore, it was logical to determine if transgenic Arabidopsis plants expressing PDC1 or ALDH2B7 using a suitable promoter would also exhibit tolerance to drought stress. An analysis of published microarray data indicated the up-regulation of the TSPO gene, which encodes an outer membrane tryptophan-rich sensory protein (TSPO), by drought stress. RT-qPCR, as well as GUS analysis of the promoter, confirmed the up-regulation of TSPO by drought stress in Arabidopsis roots and shoots. Thus, the TSPO promoter was used to drive drought-responsive expression of ALDH2B7 and PDC1. RT-qPCR analysis confirmed that the expression of PDC1 and ALDH2B7 was up-regulated, relative to WT plants, by drought stress in homozygous pTSPO-PDC1 and pTSPO-ALDH2B7 plant lines. pTSPO-ALDH2B7 and pTSPO-PDC1 transgenic lines showed prolonged survival under drought stress. Microarray analyses revealed transcriptomic changes related to metabolism in pTSPO-PDC1 plants, indicating that selective regulation of metabolism may occur; resulting in the acquisition of drought stress tolerance. These results ...Continue Reading

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Citations

Feb 7, 2019·Journal of Experimental Botany·Norma Fàbregas, Alisdair R Fernie
May 21, 2019·Frontiers in Plant Science·Yoshinori UtsumiMotoaki Seki
Mar 9, 2021·Frontiers in Plant Science·Dario Di SilvestreIrene Murgia
Jul 21, 2021·International Journal of Molecular Sciences·Velimir MladenovFederico Martinelli
Sep 16, 2021·The New Phytologist·Jürgen KreuzwieserChristiane Werner

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Datasets Mentioned

BETA
GSE110857

Methods Mentioned

BETA
transgenic
histone acetylation
acetylation
NMR
phosphotransferase
PCR

Software Mentioned

heatmapper
MapMan
PANTHER
CLC viewer
ZEN
ClustalW
GeneSpring GX

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