The modulation of the human sodium iodide symporter activity by Graves' disease sera

The Journal of Clinical Endocrinology and Metabolism
R A AjjanA P Weetman

Abstract

The transport of iodide into the thyroid, catalyzed by the Na+/I- symporter (NIS), is the initial and rate-limiting step in the formation of thyroid hormones. To study the basic characteristics of the human (h) NIS, we have established a Chinese hamster ovary cell line stably expressing the hNIS (CHO-NIS9). In agreement with previous work on the rat NIS, iodide uptake in these cells was initiated within 2 min of the addition of 131I, reaching a plateau after 30 min. Both perchlorate and thiocyanate inhibited iodide uptake in a dose-dependent manner, with inhibition evident at concentrations of 0.01 and 0.1 micromol/L, respectively, and reaching complete inhibition at 20 micromol/L and 500 micromol/L, respectively. Ouabain, which blocks the activity of the Na+/K+ adenosine triphosphatase, also inhibited iodide uptake in a dose-dependent manner, starting at concentrations of 100 micromol/L and reaching maximum inhibition at 1600 micromol/L, indicating that iodide uptake in these cells is sodium dependent. CHO-NIS9 cells were further used to study 88 sera from patients with Graves' disease, for iodide uptake inhibitory activity, which were compared with sera from 31 controls. Significant iodide uptake inhibition was taken as any i...Continue Reading

Citations

Jan 1, 2005·Indian Journal of Clinical Biochemistry : IJCB·Manorama SwainBinoy Kumar Mohanty
Aug 21, 2003·Breast Cancer Research : BCR·Peter P A Smyth
Aug 9, 2015·Journal of Endocrinological Investigation·I MullerF Grennan-Jones
Mar 7, 2001·Kidney International·C SpitzwegJ C Morris
Mar 17, 2009·International Journal of Immunogenetics·H Hadj-KacemS Péraldi-Roux
Apr 13, 2001·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·M D RingelR M Tuttle
Jan 7, 1999·Clinical Endocrinology·R A AjjanA P Weetman

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