The modulation of the symbiont/host interaction between Wolbachia pipientis and Aedes fluviatilis embryos by glycogen metabolism

PloS One
Mariana da Rocha FernandesCarlos Logullo

Abstract

Wolbachia pipientis, a maternally transmitted bacterium that colonizes arthropods, may affect the general aspects of insect physiology, particularly reproduction. Wolbachia is a natural endosymbiont of Aedes fluviatilis, whose effects in embryogenesis and reproduction have not been addressed so far. In this context, we investigated the correlation between glucose metabolism and morphological alterations during A. fluviatilis embryo development in Wolbachia-positive (W+) and Wolbachia-negative (W-) mosquito strains. While both strains do not display significant morphological and larval hatching differences, larger differences were observed in hexokinase activity and glycogen contents during early and mid-stages of embryogenesis, respectively. To investigate if glycogen would be required for parasite-host interaction, we reduced Glycogen Synthase Kinase-3 (GSK-3) levels in adult females and their eggs by RNAi. GSK-3 knock-down leads to embryonic lethality, lower levels of glycogen and total protein and Wolbachia reduction. Therefore, our results suggest that the relationship between A. fluviatilis and Wolbachia may be modulated by glycogen metabolism.

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Citations

Jan 17, 2015·International Journal of Molecular Sciences·Renato Martins da SilvaCarlos Logullo
May 18, 2016·Parasites & Vectors·Heverton Leandro Carneiro DutraLuciano Andrade Moreira
Jun 20, 2015·PloS One·Newton RuizCarlos Logullo
Dec 29, 2017·Parasites & Vectors·Gerard TerradasElizabeth A McGraw
Aug 20, 2019·Insect Biochemistry and Molecular Biology·Hai-Jian HuangXiao-Yue Hong
Sep 30, 2021·Scientific Reports·Christiano Calixto ConceiçãoCarlos Logullo
Dec 4, 2021·PLoS Neglected Tropical Diseases·M Florencia GilCorina M Berón

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Datasets Mentioned

BETA
KF517095

Methods Mentioned

BETA
transfection
ubiquitination
PCR

Software Mentioned

RNAi
dsCheck
DEQOR
Clustal W
BioEdit

Related Concepts