The modulatory effect of TLR2 on LL-37-induced human mast cells activation

Biochemical and Biophysical Research Communications
Yuan-Yuan ZhangBo Yu

Abstract

The sole and endogenous anti-microbial peptide LL-37 is a significant effector molecule in the innate host defense system. Apart from its broadly direct anti-microbial activity, the peptide also activates mast cell in respect of allergic diseases and inflammation. On the other hand, mast cell can be activated by Toll-like receptors (TLRs) which are at the center of innate immunity. It was the aim of the study to illustrate the modulatory effect of TLR2 ligands peptidoglycan (PGN) and tripalmitoyl-S-glycero-Cys-(Lys)4 (Pam3CSK4) on LL-37 induced LAD2 cells (a human mast cell line) activation. LL-37 induced LAD2 cells degranulation and the release of IL-8. TLR2 ligands didn't induce LAD2 cells degranulation, but triggered the release of IL-8. Incubation with PGN or Pam3CSK4 significantly suppressed LL-37-induced degranulation through inhibition of calcium mobilization from LAD2 cells. Similarly, the release of IL-8 was inhibited when LAD2 cells were co-stimulated with TLR2 ligands and LL-37. Studies with inhibitors of key enzymes involved in mast cell signaling indicated that the release of IL-8 induced by TLR2 ligands and LL-37 involved the activation of the PI3K, ERK, JNK and calcineurin signaling pathways. In contrast, p38 act...Continue Reading

References

Dec 31, 2003·Journal of Neuroimmunology·Theoharis C Theoharides, David E Cochrane
Sep 18, 2004·Journal of Endotoxin Research·Osamu TakeuchiShizuo Akira
Feb 25, 2006·Cell·Shizuo AkiraOsamu Takeuchi
May 24, 2006·Biochimica Et Biophysica Acta·Ulrich H N DürrAyyalusamy Ramamoorthy
Sep 6, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Amanda J Redig, Leonidas C Platanias
Nov 21, 2007·Journal of Dermatological Science·Stephen J Galli, Mindy Tsai
Mar 29, 2008·Annual Review of Immunology·Blayne A SayedMelissa A Brown
Jun 3, 2008·British Journal of Haematology·Valeska HeibMichael Stassen
Oct 22, 2008·Nature Immunology·Janet Kalesnikoff, Stephen J Galli
Jul 3, 2009·International Archives of Allergy and Immunology·Kazumi KasakuraShuichi Kaminogawa
Jul 4, 2009·The EMBO Journal·Sandra Santos-SierraPhilipp Henneke

❮ Previous
Next ❯

Citations

May 27, 2017·International Immunopharmacology·Yangyang YuBo Yu
Jun 18, 2018·International Archives of Allergy and Immunology·Bogusław NedoszytkoRoman Nowicki
Dec 4, 2019·Endocrine, Metabolic & Immune Disorders Drug Targets·Thea MagroneEmilio Jirillo
Sep 19, 2018·Vaccines·Roel M van HartenHenk P Haagsman
Feb 13, 2020·Frontiers in Medicine·Lian WangXian Jiang
Feb 13, 2018·Immunological Reviews·Gilda VarricchiBernhard F Gibbs
Feb 13, 2018·Immunological Reviews·Frank A RedegeldUlrich Blank

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Allergy and Asthma

Allergy and asthma are inflammatory disorders that are triggered by the activation of an allergen-specific regulatory t cell. These t cells become activated when allergens are recognized by allergen-presenting cells. Here is the latest research on allergy and asthma.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.