PMID: 2492657Jan 25, 1989Paper

The modulatory element TNNCT affects transcription of a Drosophila tRNA(Val)4 gene without affecting transcription complex stability

Nucleic Acids Research
F G Sajjadi, G B Spiegelman

Abstract

We have previously identified a sequence of the general form TNNCT in the 5'-flanking region of a Drosophila melanogaster tRNA(Val)4 gene which positively modulates in vitro transcription. The pentadeoxynucleotide is also present in the 5'-flank of other Drosophila tRNA genes which direct transcription efficiently in vitro. We have examined transcription of a mutant of this gene further to understand the role of the TNNCT sequence. When template competition experiments were carried out, a template which contained mutations in TNNCT was a better competitor than was the wildtype template. The time-course of transcription and the effects of temperature and ionic strength on transcription indicated that mutating the TNNCT did not alter the efficiency of stable complex formation. It is proposed that the pentadeoxynucleotide TNNCT may influence the rate of transcription initiation by RNA polymerase III bound to transcription complexes.

References

Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S K YoshinagaA J Berk
Jan 1, 1985·CRC Critical Reviews in Biochemistry·S J SharpD Söll
Apr 15, 1985·European Journal of Biochemistry·D S Louis, G B Spiegelman
Mar 25, 1983·Nucleic Acids Research·L DenteR Cortese
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S SharpD Söll
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·G CilibertoR Cortese

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