Abstract
Hemidesmosomes have been extensively studied with immunofluorescence microscopy, but owing to its limited resolution, the precise organization of hemidesmosomes remains poorly understood. We studied hemidesmosome organization in cultured keratinocytes with two- and three-color super-resolution microscopy. We observed that, in the cell periphery, nascent hemidesmosomes are associated with individual keratin filaments and that β4 integrin (also known as ITGB4) is distributed along, rather than under, keratin filaments. By applying innovative methods to quantify molecular distances, we demonstrate that the hemidesmosomal plaque protein plectin interacts simultaneously and asymmetrically with β4 integrin and keratin. Furthermore, we show that BP180 (BPAG2, also known as collagen XVII) and BP230 (BPAG1e, an epithelial splice variant of dystonin) are characteristically arranged within hemidesmosomes with BP180 surrounding a central core of BP230 molecules. In skin cross-sections, hemidesmosomes of variable sizes could be distinguished with BP230 and plectin occupying a position in between β4 integrin and BP180, and the intermediate filament system. In conclusion, our data provide a detailed view of the molecular architecture of hemid...Continue Reading
References
Mar 1, 1992·The Journal of Cell Biology·Y HiedaK Owaribe
May 1, 1990·The Journal of Investigative Dermatology·T TanakaJ R Stanley
Apr 1, 1993·The Journal of Clinical Investigation·A IshikoT Nishikawa
Apr 1, 1993·Journal of Biochemistry·Y NishizawaK Owaribe
Sep 1, 1996·The Journal of Cell Biology·B NikolicG Wiche
Apr 14, 1999·The Journal of Investigative Dermatology·L Borradori, A Sonnenberg
Oct 20, 1999·The Journal of Cell Biology·D GeertsA Sonnenberg
Jul 13, 2000·Molecular and Cellular Biology·A H SteghM E Peter
Dec 17, 2002·Journal of Cell Science·Jan KosterArnoud Sonnenberg
Dec 12, 2003·Molecular Biology of the Cell·J KosterA Sonnenberg
Jun 8, 2006·Trends in Cell Biology·Sandy H M LitjensArnoud Sonnenberg
May 26, 2007·Science·Stefan W Hell
Aug 17, 2012·The Journal of Investigative Dermatology·Kristin SeltmannThomas M Magin
Feb 11, 2015·The Journal of Investigative Dermatology·Kristin SeltmannThomas M Magin
Feb 24, 2015·Structure·Jae-Geun SongKristina Djinović-Carugo
May 29, 2015·Cell and Tissue Research·Gernot WalkoGerhard Wiche
Citations
Jan 8, 2016·Experimental Dermatology·Cristina Has
May 21, 2016·Frontiers in Cell and Developmental Biology·Arnauld Sergé
Jul 9, 2016·PloS One·Leila NahidiazarKees Jalink
Dec 15, 2017·Molecular Biology of the Cell·Naël OsmaniMichel Labouesse
Aug 10, 2016·Journal of Cell Science·Sara N StahleyAlexa L Mattheyses
Mar 8, 2018·Cell Death and Differentiation·Yan LiangYun Bai
Jun 16, 2016·F1000Research·Eric A SheldenJonathan C R Jones
Mar 14, 2019·Molecular Biology of the Cell·Mengdie WangAnne E Cress
Jan 9, 2020·The Journal of Cell Biology·Wei WangArnoud Sonnenberg
May 14, 2020·The Journal of Cell Biology·Emily I BartleAlexa L Mattheyses
Mar 4, 2017·Journal of Microscopy·G M R DE LucaE M M Manders
Feb 9, 2018·Tissue Barriers·Emily I BartleAlexa L Mattheyses
Feb 6, 2017·Cold Spring Harbor Perspectives in Biology·Karl S MatlinAki Manninen
Jan 4, 2020·European Journal of Medical Genetics·Antonio Benítez-Burraco
Dec 3, 2020·Biomolecules·Eva Roig-Rosello, Patricia Rousselle
Mar 7, 2021·International Journal of Molecular Sciences·Marcin Moch, Rudolf E Leube
Aug 2, 2020·Biochemical and Biophysical Research Communications·Gabriel T KellyTing Wang
Jul 22, 2018·Matrix Biology : Journal of the International Society for Matrix Biology·Pauline NauroyFlorence Ruggiero
May 19, 2021·Cellular Signalling·Yasaswi Gayatri Mishra, Bramanandam Manavathi