The molecular architecture of hemidesmosomes, as revealed with super-resolution microscopy

Journal of Cell Science
Leila NahidiazarKees Jalink

Abstract

Hemidesmosomes have been extensively studied with immunofluorescence microscopy, but owing to its limited resolution, the precise organization of hemidesmosomes remains poorly understood. We studied hemidesmosome organization in cultured keratinocytes with two- and three-color super-resolution microscopy. We observed that, in the cell periphery, nascent hemidesmosomes are associated with individual keratin filaments and that β4 integrin (also known as ITGB4) is distributed along, rather than under, keratin filaments. By applying innovative methods to quantify molecular distances, we demonstrate that the hemidesmosomal plaque protein plectin interacts simultaneously and asymmetrically with β4 integrin and keratin. Furthermore, we show that BP180 (BPAG2, also known as collagen XVII) and BP230 (BPAG1e, an epithelial splice variant of dystonin) are characteristically arranged within hemidesmosomes with BP180 surrounding a central core of BP230 molecules. In skin cross-sections, hemidesmosomes of variable sizes could be distinguished with BP230 and plectin occupying a position in between β4 integrin and BP180, and the intermediate filament system. In conclusion, our data provide a detailed view of the molecular architecture of hemid...Continue Reading

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Citations

Jan 8, 2016·Experimental Dermatology·Cristina Has
May 21, 2016·Frontiers in Cell and Developmental Biology·Arnauld Sergé
Dec 15, 2017·Molecular Biology of the Cell·Naël OsmaniMichel Labouesse
Aug 10, 2016·Journal of Cell Science·Sara N StahleyAlexa L Mattheyses
Jun 16, 2016·F1000Research·Eric A SheldenJonathan C R Jones
Jan 9, 2020·The Journal of Cell Biology·Wei WangArnoud Sonnenberg
May 14, 2020·The Journal of Cell Biology·Emily I BartleAlexa L Mattheyses
Mar 4, 2017·Journal of Microscopy·G M R DE LucaE M M Manders
Feb 9, 2018·Tissue Barriers·Emily I BartleAlexa L Mattheyses
Feb 6, 2017·Cold Spring Harbor Perspectives in Biology·Karl S MatlinAki Manninen
Mar 7, 2021·International Journal of Molecular Sciences·Marcin Moch, Rudolf E Leube
Aug 2, 2020·Biochemical and Biophysical Research Communications·Gabriel T KellyTing Wang
Jul 22, 2018·Matrix Biology : Journal of the International Society for Matrix Biology·Pauline NauroyFlorence Ruggiero
May 19, 2021·Cellular Signalling·Yasaswi Gayatri Mishra, Bramanandam Manavathi

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