Abstract
The molecular basis for antigenic determinants on the free beta-subunit of human chorionic gonadotrophin (hCG beta), its carboxyl-terminal peptide (hCG beta CTP) and the hCG beta-core fragment (hCG beta cf) was elucidated by means of monoclonal antibodies (MCAs). The objective of the present study was to resolve the antigenic topography of these three molecules in terms of epitope identification at different levels of structural organization as well as analysis of their spatial arrangement. An hCG beta cf preparation, a synthetic peptide corresponding to the hCG beta CTP (beta 109-145), overlapping synthetic peptides spanning the entire amino acid sequence of hCG beta, and a reduced and alkylated hCG beta preparation were assayed in a solid-phase one-site enzyme-linked immunoassay and in a soluble-phase direct-binding radioimmunoassay (RIA) or competitive RIA. The antigenic topography was mapped by incorporating the MCAs into two-site binding assays. On the surface of free hCG beta, nine different epitopes (beta 1-beta 9), arranged in three spatially distinct domains, could be distinguished. Epitopes beta 1-beta 7 were located in a single large domain on both hCG beta and the hCG beta cf whereas hCG beta CTP contained two topog...Continue Reading
Citations
Sep 27, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·P BergerUNKNOWN Members of the ISOBM TD-7 Workshop on hCG and Related Molecules
Oct 1, 1994·Immunology Today·S DirnhoferP Berger
Aug 30, 1996·Molecular and Cellular Endocrinology·A M NagyS Meuris
Dec 15, 1996·Journal of Immunological Methods·N Venkatesh, G S Murthy
Mar 4, 2000·Vaccine·A Mukhopadhyay
Dec 20, 1996·Molecular and Cellular Endocrinology·P BergerG Wick
Jun 20, 2001·Immunology·M D ChiesaT Lund
May 29, 2004·Hybridoma and Hybridomics·A A Saboor-YaraghiF Shokri
Oct 24, 2008·Human Reproduction Update·S F de Medeiros, R J Norman
Apr 4, 1998·American Journal of Reproductive Immunology : AJRI·A MukhopadhyayS S Majumdar
Oct 9, 1998·American Journal of Reproductive Immunology : AJRI·N PorakishviliT Lund
Aug 16, 2011·Reproductive Biology and Endocrinology : RB&E·Christoph ZenzmaierPeter Berger
Dec 1, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Yong-Ping TangMing Li
Sep 21, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Danielle MonniauxStéphane Fabre
Sep 24, 2014·Trends in Endocrinology and Metabolism : TEM·Peter Berger, Catharine Sturgeon
Dec 1, 2008·Expert Opinion on Medical Diagnostics·P Berger, C Sturgeon
Aug 1, 1996·European Journal of Immunology·T KlonischI M Roitt
Jul 17, 1999·British Journal of Cancer·T B ChristensenH von der Maase
Dec 22, 1999·Molecular Endocrinology·A M JacksonI Boime
Mar 30, 2013·Journal of Immunoassay & Immunochemistry·Li-Ping ZouYing-Song Wu
Apr 15, 2000·Methods : a Companion to Methods in Enzymology·S Madersbacher, P Berger
Aug 1, 1996·Journal of Reproductive Immunology·A M JacksonI M Roitt
May 1, 1998·Human Pathology·S DirnhoferP Berger
Nov 14, 2006·Molecular and Cellular Endocrinology·P BergerN Sampson