The molecular basis for the differential regulation of the hlyE-encoded haemolysin of Escherichia coli by FNR and HlyX lies in the improved activating region 1 contact of HlyX.

Microbiology
Jeffrey Green, M L Baldwin

Abstract

The regulator of fumarate and nitrate reduction (FNR) protein of Escherichia coli is an oxygen-responsive transcription regulator that acts mainly to activate the transcription of genes associated with anaerobic energy generation during periods of oxygen starvation. The hlyX gene of the swine pathogen Actinobacillus pleuropneumoniae encodes an FNR homologue, HlyX, which can complement the anaerobic respiratory deficiencies of an fnr mutant. However, FNR and HlyX have distinct but overlapping regulons because during anaerobic incubation, hlyX-expressing E. coli K-12 strains produce an otherwise latent haemolysin. The gene encoding the 'latent' haemolysin has been designated hlyE and analysis of the promoter region by DNase I footprinting reveals the presence of an FNR- (HlyX-) binding site. Anaerobic expression of an hlyE::lacZ reporter was 6.5-fold higher in hlyX compared to fnr-expressing cells. Both FNR and HlyX recruited RNA polymerase to the hlyE promoter but formed different ternary complexes. One major transcript (tsp1) initiating at 78.5 bp downstream of the FNR-binding site and four minor transcripts initiating at 73.5 (tsp2), 71.5 (tsp3), 63.5 (tsp4) and 62.5 (tsp5) bp from the FNR site were detected. From the position...Continue Reading

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Citations

Nov 4, 2010·The Journal of Microbiology·Lan Ji HuangPhil Youl Ryu
Jul 18, 2003·Chemosphere·Hiromichi Morikawa, Ozgür Cem Erkin
Jan 11, 2000·Research in Microbiology·S KokeguchiY Murayama
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Aug 5, 2004·Journal of Bacteriology·Albrecht LudwigWerner Goebel
Mar 5, 2004·Journal of Bacteriology·Neil R WybornJeffrey Green
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Jan 25, 2011·Scandinavian Journal of Infectious Diseases·Periyanaina KesikaKrishnaswamy Balamurugan
Sep 28, 2007·International Journal of Medical Microbiology : IJMM·Albrecht LudwigVolker Brade
Sep 26, 2007·Molecular Microbiology·James K LithgowJeffrey Green
Jan 15, 2010·Molecular Microbiology·Sylvain Durand, Gisela Storz
Apr 22, 2008·FEMS Microbiology Reviews·Lotte P Stenfors ArnesenPer Einar Granum
Mar 22, 2015·Microbial Pathogenesis·Anna Berthold-PlutaMonika Garbowska
Jan 30, 2008·Microbiology·Stuart HuntJeffrey Green
Sep 28, 2000·The Journal of Biological Chemistry·A AtkinsJ Green

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