The molecular characterization and immune protection of microneme 2 of Eimeria acervulina

Veterinary Parasitology
Zhenchao ZhangXiangrui Li

Abstract

Eimeria acervulina (E. acervulina) is an intestinal protozoon and few of its antigen genes were researched. In this study, the gene of E. acervulina microneme protein 2 (EaMIC2) was cloned and characterized. The degenerate primers for the conserved sequence of microneme protein 2 were designed based on Eimeria tenella microneme 2 (EtMIC2) (AF111839.1), Eimeria maxima microneme 2 (EmMIC2) (FR718971.1) and E. brunetti microneme 2 (EbMIC2) (AB723700.1) to amplify the conserved sequence of microneme protein 2. According to the conserved sequence, specific primers for the rapid amplification of cDNA ends (RACE) were designed to amplify the 3'- and 5'-ends of EaMIC2. The full length cDNA of this gene was obtained by overlapping the sequences of 3'- and 5'-extremities and amplification by reverse transcription PCR. The sequence analysis revealed that the opening reading frame (ORF) of EaMIC2 was 882bp and encoded a protein of 293 amino acids with 29.81kDa. The most part of the mature protein (base: 82-882bp, amino acid: 28-293aa) of EaMIC2 (mpmEaMIC2) was inserted into pCold TF to produce recombinant mpmEaMIC2. Western blotting assay was used to analysis the immunogenicity of mpmEaMIC2 and the result showed that the recombinant protei...Continue Reading

References

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Sep 22, 2012·Molecular and Biochemical Parasitology·Huili ZhuXiangrui Li

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Citations

Oct 28, 2016·The Journal of Eukaryotic Microbiology·ZhenChao ZhangXiangRui Li
Nov 27, 2018·Parasite : Journal De La Société Française De Parasitologie·Ming YanHui Dong
Mar 21, 2020·Parasite : Journal De La Société Française De Parasitologie·Zhenchao ZhangXiangrui Li
Dec 2, 2021·The Journal of Eukaryotic Microbiology·Huanzhi ZhaoHongyu Han

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