The Molecular Characterization and Immunity Identification of Trichomonas vaginalis Adhesion Protein 33 (AP33)

Frontiers in Microbiology
Zhenchao ZhangXiangrui Li

Abstract

Trichomoniasis is caused by Trichomonas vaginalis (T. vaginalis), which is a widespread and serious sexually transmitted pathogen in humans. The procedure of T. vaginalis adherence to the host cell is the precondition for T. vaginalis parasitism and pathogenicity. The AP33 adhesin of T. vaginalis (TvAP33) plays a key role in the process of adhesion. In this study, the specific primers for polymerase chain reaction (PCR) were designed based on the sequence of TvAP33 (GenBank Accession No. U87098.1) to amplify the open reading frame (ORF), and the ORF was inserted into pET-32a (+) to produce recombinant TvAP33 (rTvAP33). The sequence analysis indicated that the TvAP33 gene encoded a protein of 309 amino acids with 32.53 kDa, and the protein was predicted to have a high antigen index. Western blotting assay showed rTvAP33 was successfully recognized by the sera of mice experimentally infected with T. vaginalis, while native TvAP33 in the somatic extract of T. vaginalis trophozoite was as well detected by sera from rats immunized with the rTvAP33. Immunofluorescence analysis using an antibody against rTvAP33 demonstrated that the protein was expressed and located on the surface of T. vaginalis trophozoites. The recombinant protein ...Continue Reading

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Citations

Jul 20, 2021·Reports of Biochemistry & Molecular Biology·Maryam AlikhaniAsad Mirzaei

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Methods Mentioned

BETA
ELISA
PCR
confocal
Enzyme-Linked Immunosorbent Assay
electrophoresis
glycosylation

Software Mentioned

PSIpred
CLUSTALW1
BLAST
TMHMM
Motifscan
BLASTX
BLASTP
SPSS
DNASTAR
SignalP

Related Concepts

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