PMID: 6107128Sep 9, 1980Paper

The molecular forms of gamma-glutamyl transferase in bile and serum of icteric rats

Biochimica Et Biophysica Acta
M InoueY Morino

Abstract

Alterations in the molecular form of gamma-glutamyl transferase (5-glutamyl)-peptide:amino-acid 5-glutamyltransferase, EC 2.3.2.2.) were studied in the bile and serum of rats under surgical ligation of the bile duct. Polyacrylamide gel electrophoresis of the bile, followed by the enzyme stain, revealed a major, slowly migrating broad band and a minor, faster migrating band. The former was converted to the latter upon limited proteolysis of the bile with a very small amount of papain. This conversion was accompanied by a decrease in molecular size of the enzyme. Both enzyme forms were specifically adsorbed to a concanavalin A-Sepharose column. Most of the papain-treated enzyme preparation could be eluted from the column by alpha-methyl-D-glucoside, a haptenic sugar of this lectin. On the other hand, the predominant form of the enzyme in the untreated bile was eluted only in the presence both of the sugar and Triton X-100. Based on the chromatographic behavior of the two enzyme forms (detergent-solubilized and protease-solubilized form) purified from rat renal brush border membrane on concanavalin A-Sepharose column, it was concluded that the predominant form of the enzyme in the bile was the detergent-solubilized form and that t...Continue Reading

References

Nov 15, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·H TamaokiK Fujisawa
Mar 1, 1977·European Journal of Biochemistry·M InoueY Morino
Feb 10, 1978·Biochimica Et Biophysica Acta·N E Huseby
Jul 3, 1978·European Journal of Biochemistry·S HoriuchiY Morino
Apr 21, 1975·Biochimica Et Biophysica Acta·G Holdsworth, R Coleman
Aug 15, 1977·European Journal of Biochemistry·H AnttinenK I Kivirikko
Aug 1, 1968·Biological Reviews of the Cambridge Philosophical Society·G M Cook
Nov 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·L NaftalinD A Smith
Nov 1, 1960·Archives of Biochemistry and Biophysics·J A GOLDBARGA M RUTENBURG

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Citations

Apr 5, 2005·Toxicologic Pathology·Philip F Solter
Jul 1, 1983·Annals of Clinical Biochemistry·M MasuikeG Kosaki
Sep 1, 1981·Acta paediatrica Scandinavica·B DalensB Dastugue

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