PMID: 1204215Dec 1, 1975Paper

The molecular heterogeneity and instability of radioiodinated human chorionic gonadotrophin

Clinical Biochemistry
B W Griffiths, A Godard

Abstract

Gel chromatographic studies on radioiodinated HCG have shown that a polymer and fragmented materials are associated with the radiolabelled product which interfere in the RIA of HCG in serum. Scatchard plots of experimental data indicated that the polymer material (fraction I) has two species of antigenic determinants that recognize two species of binding sites on the anti-HCG-beta. The use of purified 125I-HCG (gel chromatographic fraction II) for radioligand improved the reliability of the RIA. It was further demonstrated that a labelled preparation of HCG could be used for RIA tests for periods of five weeks or longer provided that chromatographic purification was carried out immediately prior to the assay.

References

Jul 15, 1972·American Journal of Obstetrics and Gynecology·J L VaitukaitisG T Ross
Feb 1, 1967·The Journal of Clinical Investigation·W D OdellP L Rayford
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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Citations

Jan 1, 1980·The International Journal of Biochemistry·J C Fretz, J C Gan
May 19, 2007·Biochimica Et Biophysica Acta·Melvin E KlegermanDavid D McPherson
Jan 16, 1985·Biochemical and Biophysical Research Communications·W WeberP C Heinrich

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