The molecular identity of fleas (Siphonaptera) carrying Rickettsia felis, Bartonella clarridgeiae and Bartonella rochalimae from dogs and cats in Northern Laos

Heliyon
Nichola E D CalvaniJan Šlapeta

Abstract

Cat fleas (Ctenocephalides felis) are the most commonly recognised ectoparasites of domestic pets globally and are frequently implicated in the transmission of a variety of zoonotic vector-borne pathogens. The aim of the present study was to investigate the morphological and molecular identity of fleas parasitising cats and dogs in Northern Laos and screen them for a range of bacterial pathogens. Fleas (n = 120) were collected from dogs and cats and morphologically identified as Ctenocephalides felis (115/120), Ctenocephalides orientis (4/120) and Pulex irritans (1/120). Molecular barcoding using the cytochrome c oxidase subunit I gene (cox1) was used to confirmed species identity of 21 selected fleas. The cat flea (C. felis) was the most dominant flea identified. Rickettsia and Bartonella spp. DNA was detected in 21/21 and 7/21 samples, respectively, via a multiplex real-time PCR targeting gltA and ssrA. Sequencing of the seven Bartonella-positive samples and ten Rickettsia-positive samples revealed Bartonella clarridgeiae, Bartonella rochalimae, Rickettsia felis and Rickettsia sp. genotype RF2125 DNA. Anaplasma platys DNA was detected in a single C. felis after 20 of the 21 DNA samples were screened using a commercial PCR pan...Continue Reading

References

Jul 16, 2003·Annals of the New York Academy of Sciences·P ParolaD Raoult
Feb 24, 2006·Emerging Infectious Diseases·Simaly PhongmanyPaul N Newton
Apr 21, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E AngelakisJ M Rolain
May 15, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M VaragnolD Raoult
Sep 19, 2009·Journal of Veterinary Internal Medicine·D D CarradeJ E Sykes
Apr 10, 2010·Transactions of the Royal Society of Tropical Medicine and Hygiene·Bounkong SyhavongPaul N Newton
Sep 6, 2011·Annual Review of Entomology·Rebecca J Eisen, Kenneth L Gage
Dec 14, 2011·Comparative Immunology, Microbiology and Infectious Diseases·Tahar KernifPhilippe Parola
Feb 20, 2014·Medical and Veterinary Entomology·A L LawrenceJ Šlapeta
Jul 26, 2014·Emerging Infectious Diseases·Sabine DittrichDaniel H Paris
May 11, 2016·Parasites & Vectors·Tawin InpankaewRebecca J Traub
May 23, 2020·Emerging Infectious Diseases·Vito ColellaLénaïg Halos

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Citations

Sep 4, 2021·Veterinary Parasitology : Regional Studies and Reports·Phirabhat SaengsawangTawin Inpankaew

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Datasets Mentioned

BETA
MT394897-MT394913

Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

CLC Main Workbench
CFX Maestro

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