The molecular mass and isoelectric point of plant proteomes

BMC Genomics
Tapan Kumar MohantaAhmed Al-Harrasi

Abstract

Cell contain diverse array of proteins with different molecular weight and isoelectric point (pI). The molecular weight and pI of protein play important role in determining the molecular biochemical function. Therefore, it was important to understand the detail regarding the molecular weight and pI of the plant proteins. A proteome-wide analysis of plant proteomes from 145 species revealed a pI range of 1.99 (epsin) to 13.96 (hypothetical protein). The spectrum of molecular mass of the plant proteins varied from 0.54 to 2236.8 kDa. A putative Type-I polyketide synthase (22244 amino acids) in Volvox carteri was found to be the largest protein in the plant kingdom. However, Type-I polyketide synthase was not found in higher plant species. Titin (806.46 kDa) and misin/midasin (730.02 kDa) were the largest proteins identified in higher plant species. The pI and molecular weight of the plant proteins showed a trimodal distribution. An acidic pI (56.44% of proteins) was found to be predominant over a basic pI (43.34% of proteins) and the abundance of acidic pI proteins was higher in unicellular algae species relative to multicellular higher plants. In contrast, the seaweed, Porphyra umbilicalis, possesses a higher proportion of basic...Continue Reading

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Citations

Apr 11, 2020·PloS One·Tapan Kumar MohantaAhmed Al-Harrasi
Mar 7, 2021·International Journal of Molecular Sciences·Magda L Arce-RodríguezNeftalí Ochoa-Alejo
Mar 25, 2021·Scientific Reports·Tapan Kumar MohantaAhmed Al-Harrasi
Apr 20, 2021·Journal, Genetic Engineering & Biotechnology·Anila HodaEnkelejda Sallaku
Nov 3, 2021·IMA Fungus·Tapan Kumar Mohanta, Ahmed Al-Harrasi

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Methods Mentioned

BETA
PCA

Software Mentioned

BLASTP
Unscrambler
Protein isoelectric point calculator ( IPC Python )
excel
Linux

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