The molecular mechanism and effect of cannabinoid-2 receptor agonist on the blood-spinal cord barrier permeability induced by ischemia-reperfusion injury

Brain Research
Ming-chao YangYan-feng Wang

Abstract

Previous studies have shown that modulation of the receptor-mediated endocannabinoid system during ischemia injury can induce potent neuroprotective effects. However, little is known about whether cannabinoid-2 (CB2) receptor agonist would produce a protective effect on blood-spinal cord barrier (BSCB) during ischemia. Using an in vivo transient spinal cord ischemia model in rats, JWH-015 (1mg/kg, i.p.), a CB2 receptor selective agonist, or vehicles were injected 20 min before ischemia. The effects of JWH-015 on BSCB permeability, the major structural protein for the formation of caveolae, caveolin-1 (cav-1), tight junction (TJ) protein Occludin and zona occludens protein-1 (ZO-1) were examined at day 1, day 3 and day 7 of reperfusion after transient spinal cord ischemia in rats. Here we demonstrated that JWH-015 significantly down-regulated the expression of cav-1, up-regulated the expression of TJ proteins, and then decreased the permeability of BSCB compared with control group. In addition, using an in vitro BBB model, oxygen glucose deprivation (OGD) was applied to simulate spinal cord ischemia in vitro in Human brain microvascular endothelial cells (HBMECs). JWH-015 greatly increased the transepithelial electrical resistan...Continue Reading

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Citations

Mar 4, 2020·International Journal of Molecular Sciences·Michael EndersStefanie Kuerten
Apr 17, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ronald J EllisJenny Iudicello
Feb 9, 2018·Tissue Barriers·Lorenza González-MariscalChristian Hernández-Guzmán
Jun 3, 2021·International Journal of Molecular Sciences·Ludmila A KasatkinaEva M Sturm

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