The molecular mechanism of autoxidation for human oxyhemoglobin. Tilting of the distal histidine causes nonequivalent oxidation in the beta chain.

The Journal of Biological Chemistry
Mie TsurugaK Shikama

Abstract

Human oxyhemoglobin showed a biphasic autoxidation curve containing two rate constants, i.e. kf for the fast autoxidation due to the alpha chains, and ks for the slow autoxidation of the beta chains, respectively. Consequently, the autoxidation of the HbO2 tetramer produces two different curves from the pH dependence of kf and ks. The analysis of these curves revealed that the beta chain of the HbO2 tetramer does not exhibit any proton-catalyzed autoxidation, unlike the alpha chain, where a proton-catalyzed process involving the distal histidine residue can play a dominant role in the autoxidation rate. When the alpha and beta chains were separated from the HbO2 tetramer, however, each chain was oxidized much more rapidly than in the tetrameric parent. Moreover, the separated beta chain was recovered completely to strong acid catalysis in its autoxidation rate. These new findings lead us to conclude that the formation of the alpha1beta1 contact produces in the beta chain a conformational constraint whereby the distal histidine at position 63 is tilted away slightly from the bound dioxygen, preventing the proton-catalyzed displacement of O-2 by a solvent water molecule. The beta chains have thus acquired a delayed autoxidation i...Continue Reading

References

Nov 15, 1978·European Journal of Biochemistry·K Shikama, Y Sugawara
Aug 1, 1976·Journal of Biochemistry·T Gotoh, K Shikama
Sep 13, 1985·Journal of Chromatography·S M Turci, M J McDonald
Jul 1, 1973·Analytical Biochemistry·R C Williams, K Y Tsay
Nov 20, 1973·Biochemistry·A Mansouri, K H Winterhalter
Apr 23, 1974·Biochemical and Biophysical Research Communications·W J WallaceW S Caughey
Apr 28, 1967·Journal of Molecular Biology·M A Rosemeyer, E R Huehns
Sep 1, 1980·European Journal of Biochemistry·Y Sugawara, K Shikama
May 25, 1983·Journal of Molecular Biology·E R HenryW A Eaton
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D A ChernoffA W Steele
Apr 15, 1982·Nature·B Shaanan
Jan 1, 1994·Methods in Enzymology·J M RifkindJ Heim
May 1, 1994·Biological Reviews of the Cambridge Philosophical Society·K Shikama, A Matsuoka
Jan 1, 1993·The Japanese Journal of Physiology·Y SugawaraH Sakamoto
Jan 4, 1997·Biochimica Et Biophysica Acta·M Tsuruga, K Shikama

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May 26, 2009·Biochemistry·Alexandra IoanoviciuPaul R Ortiz de Montellano
Jan 11, 2002·European Journal of Biochemistry·Jun pei YasudaKeiji Shikama
Mar 26, 2003·European Journal of Biochemistry·Satoshi KamimuraKeiji Shikama
Oct 3, 2012·Antioxidants & Redox Signaling·Cornelius L VarnadoJohn S Olson
Dec 4, 2012·Antioxidants & Redox Signaling·Celia BonaventuraAlvin L Crumbliss
Jul 27, 2011·PloS One·Rolf H BremmerMaurice C G Aalders
Dec 15, 2004·Critical Reviews in Biochemistry and Molecular Biology·Keiji Shikama, Ariki Matsuoka
Mar 9, 2011·International Journal of Biological Macromolecules·Phakawat TongnuanchanPonusa Songtipya
Aug 24, 2010·Forensic Science International : Synergy·Rolf H BremmerMaurice C G Aalders
Feb 2, 2008·Clinica Chimica Acta; International Journal of Clinical Chemistry·Junya Ono, Hajime Yoshimura
Aug 28, 2003·Acta Physiologica Scandinavica·Y SugawaraH Soda
Jan 22, 2015·Chemical Reviews·Yue YuanChien Ho
Oct 23, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Dominik DobrunzCornelia G Palivan
Jun 15, 2018·Animal Science Journal = Nihon Chikusan Gakkaihō·Jin WangYong-Qing Zeng
Aug 23, 2002·The Journal of Biological Chemistry·Gen KobayashiKeiji Shikama
Dec 21, 2006·The Analyst·Vanesa SanzJavier Galbán
Jan 11, 2020·Biotechnology Progress·Clayton CuddingtonAndre Palmer
Aug 12, 2006·The Journal of Biological Chemistry·Suiping ZhouAndrew J Gow
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