The molecular mechanism of phase variation of H. influenzae lipopolysaccharide.

Cell
J N WeiserE R Moxon

Abstract

Multiple carbohydrate structures on the outer-membrane lipopolysaccharide (LPS) of the gram-negative pathogen H. influenzae undergo high frequency, reversible loss, indicative of phase variation. Characterization of a genetic locus, lic-1, responsible for expression of two LPS epitopes displaying phase variation, showed it to comprise four genes. The first gene mediates phase variation. At its 5' end, within the open reading frame, are a variable number of tandem repeats of the tetramer CAAT. By shifting upstream initiation codons in or out of frame, these 4 bp units create a translational switch. The phenotype of organisms corresponds to the number of 4 bp units. Phase variation between three levels of expression ( +, +, and -) of lic-1-derived epitopes is caused by differences in the three phases of translation of the 5' terminus of this gene. Phase variation also allows for selection of organisms displaying certain LPS epitopes in vivo.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1978·The Journal of Infectious Diseases·A R Flesher, R A Insel
Jul 1, 1988·Reviews of Infectious Diseases·J M GriffissM A Apicella
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S K HoisethR P Silver
Jan 1, 1986·Cold Spring Harbor Symposia on Quantitative Biology·K MullisH Erlich
Feb 1, 1986·Analytical Biochemistry·M Hattori, Y Sakaki
Jun 1, 1985·Southern Medical Journal·T P Blair, W P Baker
Jan 1, 1984·Annual Review of Biochemistry·P H von HippelJ A McSwiggen
Oct 25, 1980·Journal of Molecular Biology·F SangerB A Roe
Apr 5, 1981·Journal of Molecular Biology·A OkaM Takanami
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Jan 11, 1984·Nucleic Acids Research·R Staden
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M D BigginG F Hong
Jan 1, 1982·Annual Review of Microbiology·S Michaelis, J Beckwith

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Citations

Apr 1, 1995·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M RahmanA Krook
Jul 30, 2008·Antonie van Leeuwenhoek·Florence Wisniewski-Dyé, Ludovic Vial
Oct 25, 2012·Glycoconjugate Journal·Rosanna E B Young, Derek W Hood
Dec 1, 1992·Trends in Genetics : TIG·B D Robertson, T F Meyer
Jul 4, 1995·Gene·G A TruebaR L Zuerner
Jul 1, 1991·Research in Microbiology·D J MaskellE R Moxon
Mar 1, 1994·Research in Microbiology·J T Belisle, P J Brennan
Jan 28, 2003·Progress in Lipid Research·Christian SohlenkampOtto Geiger
Jul 21, 1999·Molecular and Biochemical Parasitology·K W DeitschT E Wellems
Nov 14, 1997·Trends in Genetics : TIG·C M TangE R Moxon
Feb 1, 2002·FEMS Immunology and Medical Microbiology·Kate L SeibMichael P Jennings
Jan 1, 1994·Current Biology : CB·E R MoxonR E Lenski
Oct 25, 2000·Trends in Microbiology·J N Weiser
Feb 5, 2003·Trends in Microbiology·Ruth C Massey, Angus Buckling
Oct 6, 2001·Current Opinion in Microbiology·G J McKenzie, S M Rosenberg
May 7, 1998·Molecular Microbiology·N J SaundersE R Moxon
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W HoodE R Moxon
Jul 7, 1998·The Journal of Experimental Medicine·E LünebergM Frosch
Jul 9, 2004·The Journal of Experimental Medicine·Janine ZweignerElaine I Tuomanen
Apr 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·J C PatonR Morona
Jul 3, 1998·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·J N Weiser
May 30, 2013·The Journal of Infectious Diseases·Jessica PooleMichael A Apicella
Jul 30, 2009·Nucleic Acids Research·Yoshinao AzumaMutsunori Shirai

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