The molecular mechanisms of XBP-1 gene silencing on IRE1α-TRAF2-ASK1-JNK pathways in oral squamous cell carcinoma under endoplasmic reticulum stress

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Hai-Ying ChenLexin Wang

Abstract

Proteasome inhibitor Carbobenzoxy-Leu-Leu-leucinal (MG132) induces the unfolded protein response (UPR) in oral squamous cell carcinoma (OSCC). X-box binding protein 1 (XBP1) is a key UPR component that regulates endoplasmic reticulum stress (ER) homeostasis. This study was aimed to investigate the activation of IRE1α-TRAF2-ASK1-JNK pathway by silencing the XBP1 expression in an OSCC cell line. The XBP1 specific short hairpin RNA (shRNA) plasmid vector was constructed and then transfected into the Tca-8113 cells. The effect of XBP-1 gene silencing on IRE1α-TRAF2-ASK1-JNK pathway under MG132 induced endoplasmic reticulum stress in Tca-8113 were investigated by real-time RT-PCR or western blot. Cell apoptosis was detected by flow cytometry. XBP1 expression was reduced in transfected groups and MG132 groups. shRNA-XBP1 induces IRE1α-TRAF2-ASK1 signaling activation to activate pro-apoptotic ASK1-JNK signaling. Moreover, combined shRNA-XBP1 with MG132 further enhanced downregulated XBP1 expression and upregulated activation of ASK1-JNK signaling. Silencing XBP1 expression under MG132 induced ER stress block the XBP1 survival pathway and synergism with MG132 to promote Tca8113 cell apoptosis. These findings provide a therapeutic optio...Continue Reading

References

Jul 22, 2011·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shuang-feng ChenLe-Xin Wang
Aug 21, 2012·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hai-Ying ChenLexin Wang
Feb 8, 2013·Cold Spring Harbor Perspectives in Biology·Brooke M GardnerPeter Walter
Sep 3, 2013·Biochemical and Biophysical Research Communications·Xiucheng LiChunping Zou
Feb 6, 2014·Cancer Biology & Therapy·Beth Overley-AdamsonJames D Thacker
May 3, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jing LiuJianping Cao
Aug 6, 2014·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Hai-ying ChenLe-xin Wang

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Citations

Oct 21, 2016·Environmental Science and Pollution Research International·Miao LongJianbin He
Feb 17, 2017·Cellular Microbiology·Nilanka PereraNicole Zitzmann
Jun 24, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ioannis Gkouveris, Nikolaos G Nikitakis
Apr 13, 2019·International Journal of Molecular Sciences·Nobumasa TakasugiTakashi Uehara
Jun 4, 2019·International Journal of Molecular Sciences·Olivier Pluquet, Antoine Galmiche
Oct 9, 2018·Frontiers in Immunology·Sining ZhuPing Xie
Dec 1, 2017·Cancer Chemotherapy and Pharmacology·Margot S F RoetenGerrit Jansen
Apr 16, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shanshan ChenXiaoli Zhu
Feb 24, 2021·Nature Reviews. Cardiology·Jun RenYingmei Zhang
Aug 29, 2021·Diabetes Research and Clinical Practice·Xiang RenHui Kong

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