The molecular path to in vitro shoot regeneration

Biotechnology Advances
Hans MotteStefaan Werbrouck

Abstract

Plant regeneration through de novo shoot organogenesis in tissue culture is a critical step in most plant transformation and micropropagation procedures. Establishing an efficient regeneration protocol is an empirical process and requires optimization of multiple factors that influence the regeneration capacity. Here, we review the molecular process of shoot induction in a two-step regeneration protocol and focus on the role of auxins and cytokinins. First, during incubation on an auxin-rich callus induction medium (CIM), organogenic callus is produced that exhibits characteristics of a root meristem. Subsequent incubation on a cytokinin-rich shoot induction medium (SIM) induces root to shoot conversion. Through a detailed analysis of the different aspects of shoot regeneration, we try to reveal hinge points and novel candidate genes that may be targeted to increase shoot regeneration capacity in order to improve transformation protocols.

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Citations

Aug 11, 2015·Plant Science : an International Journal of Experimental Plant Biology·Adeyemi O AremuJohannes Van Staden
Aug 11, 2015·Plant Science : an International Journal of Experimental Plant Biology·Or ShemerLeor Eshed Williams
Jun 30, 2015·The Plant Journal : for Cell and Molecular Biology·Yan XuLizhong Xiong
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May 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hans MotteDanny Vereecke
Nov 28, 2018·Journal of Experimental Botany·Hans Motte, Tom Beeckman
Sep 11, 2019·Journal of Experimental Botany·Jinwoo ShinPil Joon Seo
Oct 24, 2019·Journal of Experimental Botany·Carlos Hernán Barrera-RojasFabio Tebaldi Silveira Nogueira
Jun 23, 2015·Plant Signaling & Behavior·Wenzislava Ckurshumova, Thomas Berleth
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Sep 30, 2020·Plants·Robin Lardon, Danny Geelen
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Aug 28, 2020·Biomolecules·Adeyemi O AremuKarel Doležal
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Aug 28, 2021·International Journal of Molecular Sciences·Martin RasporTatjana Ćosić
Sep 22, 2021·Annual Review of Genetics·Leor Eshed Williams

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