Aug 23, 2005

The mono-ADP-ribosyltransferases Alt and ModB of bacteriophage T4: target proteins identified

Biochemical and Biophysical Research Communications
Reinhard DeppingWolfgang Rüger


Infection of Escherichia coli by bacteriophage T4 leads to the expression of three phage mono-ADP-ribosyltransferases (namely, Alt, ModA, and ModB), each of which modifies a distinct group of host proteins. To improve understanding of these interactions and their consequences for the T4 replication cycle, we used high-resolution two-dimensional gel electrophoresis and mass-spectrometry to identify some of the putative target proteins ADP-ribosylated in vitro by Alt (total approximately 27) and ModB (total approximately 8). E. coli trigger factor and the elongation factor EF-Tu were 2 targets of ModB action, and these proteins were among the 10 identified as targets of Alt, hinting that these factors are involved in phage replication.

Mentioned in this Paper

Metabolic Process, Cellular
Formicum acidum, formic acid, Homeopathic preparation
Drug Delivery Systems
HSPD1 gene
Alkalescens-Dispar Group
Elongation Factor
Enzymes, antithrombotic
DNA-Directed RNA Polymerase
RNA Polymerase Assembly Pathway
Complex (molecular entity)

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.