The Moraxella IgD-binding protein MID/Hag is an oligomeric autotransporter

Microbes and Infection
Teresia HallströmKristian Riesbeck

Abstract

The immunoglobulin D (IgD)-binding protein MID/Hag of the human respiratory pathogen Moraxella catarrhalis is an outer membrane protein of approximately 200kDa belonging to the autotransporter family. MID also functions as an adhesin and hemagglutinin. In the present paper, the ultrastructure of MID was mapped. Using a series of Escherichia coli transformants, the last 210 aa of the C-terminal region were shown to translocate protein MID through the outer membrane suggesting that MID has a beta-barrel structure comprising of 10 transmembrane beta-sheets. Electron microscopy mapping with gold-labelled specific antibodies, and partial unravelling using guanidine hydrochloride showed that the rest of the MID protein forms an approximately 120nm long, fibrillar structure in which the individual monomers fold back on themselves to expose a globular distal domain at their tips comprising both the IgD-binding (MID962-1200) and adhesive (MID764-913) regions. This positions their N-termini close to the C-terminal membrane spanning domains. Mass measurements by scanning transmission electron microscopy (STEM) verified that the MID molecule is an oligomer.

References

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Citations

Jan 8, 2009·The Journal of Infectious Diseases·Elena RonanderKristian Riesbeck
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Stefan P W de VriesPeter W M Hermans
Jan 22, 2010·PLoS Pathogens·Maria Laura A Perez VidakovicsKristian Riesbeck
Jun 5, 2015·Human Vaccines & Immunotherapeutics·Oindrilla MukherjeeKristian Riesbeck
Dec 4, 2014·International Journal of Medical Microbiology : IJMM·Birendra SinghKristian Riesbeck
Apr 3, 2013·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Timothy F MurphyMelinda M Pettigrew
Nov 30, 2018·The Journal of Infectious Diseases·Timothy F MurphyHervé Tettelin
Apr 7, 2009·Current Protocols in Immunology·Sarah M AndrewRichard Coico
May 2, 2009·Current Opinion in Infectious Diseases·M Laura Perez Vidakovics, Kristian Riesbeck
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Johan JendholmKristian Riesbeck

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