The morphological and immunohistochemical analysis of renal biopsies by light and electron microscopy using a single processing method.

The Histochemical Journal
A L BowdlerG R Newman

Abstract

A methodology is described in which a number of well-established research techniques are brought together to enable the complete diagnostic analysis of a renal biopsy on a single piece of tissue. By embedding the biopsy in the acrylic resin LR White, unsupported sections of which are stable in the electron beam, light and electron microscopy and immunocytochemistry become feasible on sections from the same block. The biopsy is glutaraldehyde fixed but post-fixation in osmium tetroxide, which is often deleterious to antigen preservation, is omitted. Extraction in organic solvents and resin monomer is minimized by rapidly infiltrating the tissue from 70% ethanol and polymerizing the resin catalytically at 0 degrees C. Semithin sections can be stained with haematoxylin and eosin, Toluidine Blue or methenamine silver, giving results similar or superior to those obtained from paraffin sections. Thin sections show that the standard of morphological preservation is similar to that seen using epoxide sections even though the kidney is unosmicated. The tissue retains a high level of antigen reactivity, which, in the limited number of cases so far examined, has paralleled or exceeded that demonstrated by conventional immunofluorescence o...Continue Reading

References

Sep 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D G BaskinJ A Parsons
Jan 1, 1986·Cell and Tissue Research·N YoshimuraG R Newman
Sep 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G R Newman, J A Hobot
Feb 1, 1987·The Histochemical Journal·G R Newman
Jan 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Bendayan, M Zollinger

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Citations

Jan 1, 1989·Current Opinion in Immunology·J E Beesley
Oct 1, 1996·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Jun 1, 1997·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Mar 11, 1999·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Jun 1, 1993·Neuropathology and Applied Neurobiology·S K SinghraoG R Newman
Feb 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·S H BrorsonF Skjørten
May 17, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Amanda V AlbuquerqueHélio Chiarini-Garcia
Aug 12, 2016·Indian Journal of Pathology & Microbiology·Swasti ShubhamHimansu Sekhar Mahapatra
Jun 17, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R Y OsamuraA Matsuno
Jun 1, 1989·Calcified Tissue International·D H CarterJ E Aaron
Mar 11, 2000·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·S H Brorson

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