Jul 1, 1989

The morphological and immunohistochemical analysis of renal biopsies by light and electron microscopy using a single processing method

The Histochemical Journal
A L BowdlerG R Newman

Abstract

A methodology is described in which a number of well-established research techniques are brought together to enable the complete diagnostic analysis of a renal biopsy on a single piece of tissue. By embedding the biopsy in the acrylic resin LR White, unsupported sections of which are stable in the electron beam, light and electron microscopy and immunocytochemistry become feasible on sections from the same block. The biopsy is glutaraldehyde fixed but post-fixation in osmium tetroxide, which is often deleterious to antigen preservation, is omitted. Extraction in organic solvents and resin monomer is minimized by rapidly infiltrating the tissue from 70% ethanol and polymerizing the resin catalytically at 0 degrees C. Semithin sections can be stained with haematoxylin and eosin, Toluidine Blue or methenamine silver, giving results similar or superior to those obtained from paraffin sections. Thin sections show that the standard of morphological preservation is similar to that seen using epoxide sections even though the kidney is unosmicated. The tissue retains a high level of antigen reactivity, which, in the limited number of cases so far examined, has paralleled or exceeded that demonstrated by conventional immunofluorescence o...Continue Reading

  • References6
  • Citations15

References

  • References6
  • Citations15

Citations

Mentioned in this Paper

Immunofluorescence Assay
Sample Fixation
Immunocytochemistry
Kidney
Hexamine Silver
Eosine Yellowish
Tolonium chloride
Organic solvent product
Infiltration
Embedding

About this Paper

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