The morphology and cytochemistry of human T-cell subpopulations defined by monoclonal antibodies and Fc receptors

British Journal of Haematology
R J ArmitageJ C Cawley

Abstract

Leu 2a+ (suppressor/cytotoxic) and Leu 3a+ (helper/inducer) T-cell subsets of normal peripheral blood lymphocytes, purified by fluorescence-activated cell sorting, respectively showed 50 +/- 22% (range 31-92%) and 82 +/- 9% (range 69-93%) dot non-specific esterase (NSE) and 48 +/- 20% (range 31-91%) and 79 +/- 10% (range 64-92%) dot acid-phosphatase (APase) staining. Mature T cells defined by monoclonal antibody (UCHT1) displayed 81% and 79% dot NSE and APase positivity, while E-rosetting cells not staining with UCHT1 showed only 15% and 10% dot-positivity and were generally larger cells with more abundant cytoplasm. Examination of Fc-rosetting cells within the different T-cell subsets showed that dot positivity was not directly related to microFc receptor (microFcR) expression; particularly among Leu 2a+ lymphocytes, many microFcR+ cells lacked dot staining. It is concluded that dot staining for hydrolytic enzymes is a marker of true T-cells (as defined by monoclonal antibodies), although a minority of such cells (approximately 20%) lack this staining pattern. Although Leu 3a+ cells display a higher percentage of dot positivity than do Leu 2a+ cells, the difference is not clinically useful, and the two cell populations are not...Continue Reading

References

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Citations

Sep 30, 1983·Journal of Immunological Methods·A T FranziC E Grossi
Jan 1, 1991·Electron Microscopy Reviews·I Vollenweider, P Groscurth
Sep 1, 1988·Acta Neurologica Scandinavica·J De Reuck, P Vanderdonckt
Feb 1, 1983·British Journal of Haematology·J Bernard, J Dufer
Apr 1, 1985·British Journal of Haematology·R J Armitage, J C Cawley
Aug 1, 1984·Scandinavian Journal of Dental Research·A C JohannessenN Skaug
Jan 1, 1989·Indian Journal of Pediatrics·M JayasheelaS N Saxena
Sep 1, 1993·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·L Kass
May 1, 1983·Scandinavian Journal of Haematology·A Crockard, D Catovsky
Apr 1, 1985·American Journal of Hematology·R J ArmitageJ C Cawley
Feb 1, 1984·Scandinavian Journal of Haematology·A HirtH P Wagner

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