The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy

Chemical Science
Di ZhangJianfang Wang

Abstract

The cellular uptake efficiency of nanostructures has been demonstrated to be highly dependent on the surface charge, size and shape although the cellular internalization process is still far from being well-understood. In this work, a series of NaYF4:Yb3+, Er3+ upconversion nanoparticles (UCNPs) with different morphologies and surface coatings were prepared to explore the influence of surface charge and morphology on the cellular internalization process with single-particle fluorescence microscopy. It is found that the higher the surface charge and larger the surface-to-volume ratio of the nanoparticles, the more efficient the cellular uptake will be. Particularly, the surface charge is demonstrated to be the primary influence factor for small sized nanoparticles on the cellular uptake process. By blocking the endocytosis routes with temperature modulation (from 37 to 4 °C) or introduction of chemical inhibitors (dynasore and genistein), multiplexed mechanisms are found to be involved in the cellular uptake process, including clathrin- and caveolae-mediated endocytosis, physical adhesion and penetration, and so on. Moreover, in the aspect of size effect, an energy-dependent endocytosis process plays a more important role for la...Continue Reading

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Citations

Feb 23, 2019·Analytical and Bioanalytical Chemistry·Yuanyuan MaLehui Xiao
Mar 18, 2020·Chemical Communications : Chem Comm·Rohit BhadoriaPavel Starkov
Mar 11, 2020·Pharmaceutics·Catarina Roma-RodriguesAlexandra R Fernandes
Mar 25, 2019·International Journal of Molecular Sciences·Kyujin ShinKang Taek Lee
Jan 26, 2020·Archives of Pharmacal Research·Xuan Thien Le, Yu Seok Youn
Nov 1, 2020·International Journal of Molecular Sciences·Parinaz SabourianAshok Kakkar
Nov 22, 2019·Analytical Chemistry·Zhongju YeLehui Xiao
Jan 7, 2021·ACS Applied Materials & Interfaces·Bing ChenFeng Wang
Sep 23, 2021·Molecular Pharmaceutics·Michele DullySarah P Hudson
Dec 9, 2021·ACS Biomaterials Science & Engineering·Liying XiaoDavid L Kaplan
Dec 17, 2021·Applied Microbiology and Biotechnology·Graciela Dolores Avila-QuezadaMahendra Rai
Jan 7, 2022·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Vaidas KlimkeviciusVitalijus Karabanovas

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Methods Mentioned

BETA
transmission
scanning electron microscopy
fluorescence microscopy
infrared spectroscopy
dynamic light scattering
fluorescence imaging
Fluorescence

Software Mentioned

ImageJ

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