The most polymorphic residue on Plasmodium falciparum apical membrane antigen 1 determines binding of an invasion-inhibitory antibody.

Infection and Immunity
Andrew M ColeyMichael Foley

Abstract

Apical membrane antigen 1 (AMA1) is currently one of the leading malarial vaccine candidates. Anti-AMA1 antibodies can inhibit the invasion of erythrocytes by Plasmodium merozoites and prevent the multiplication of blood-stage parasites. Here we describe an anti-AMA1 monoclonal antibody (MAb 1F9) that inhibits the invasion of Plasmodium falciparum parasites in vitro. We show that both reactivity of MAb 1F9 with AMA1 and MAb 1F9-mediated invasion inhibition were strain specific. Site-directed mutagenesis of a fragment of AMA1 displayed on M13 bacteriophage identified a single polymorphic residue in domain I of AMA1 that is critical for MAb 1F9 binding. The identities of all other polymorphic residues investigated in this domain had little effect on the binding of the antibody. Examination of the P. falciparum AMA1 crystal structure localized this residue to a surface-exposed alpha-helix at the apex of the polypeptide. This description of a polymorphic inhibitory epitope on AMA1 adds supporting evidence to the hypothesis that immune pressure is responsible for the polymorphisms seen in this molecule.

References

Aug 20, 1976·Science·W Trager, J B Jensen
Nov 15, 1996·The Journal of Biological Chemistry·A N HodderR F Anders
Nov 15, 2000·Methods in Enzymology·S S SidhuJ A Wells
Apr 9, 2001·Infection and Immunity·A N HodderR F Anders
Dec 17, 2003·The Journal of Biological Chemistry·Olivier SilvieDominique Mazier
Jan 27, 2004·Infection and Immunity·Joanne L CaseyMichael Foley
Feb 26, 2004·Thrombosis and Haemostasis·Christiane MühleHolm Schneider
Feb 26, 2005·Science·Juan Carlos PizarroGraham A Bentley
Aug 17, 2005·Molecular Microbiology·Steven A HowellMichael J Blackman
Sep 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tao BaiAdrian H Batchelor
Sep 13, 2005·Molecular and Biochemical Parasitology·Marie-Laure Chesne-SeckGraham A Bentley
Jul 14, 2006·Molecular and Biochemical Parasitology·Federica VerraDavid J Conway

❮ Previous
Next ❯

Citations

Jun 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·Junhui DuanLouis Miller
Jul 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Michelle J BoyleJames G Beeson
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Prakash SrinivasanLouis H Miller
Jan 24, 2009·The Journal of Biological Chemistry·Karen S HarrisMichael Foley
Apr 22, 2008·The Journal of Infectious Diseases·James G BeesonMirja Hommel
Jan 13, 2009·Clinical Microbiology Reviews·Denise L DoolanJ Kevin Baird
Jun 8, 2010·Malaria Journal·Danny W WilsonJames G Beeson
Oct 3, 2007·PLoS Pathogens·Andrew M ColeyAdrian H Batchelor
Jan 24, 2009·PLoS Pathogens·Christine R CollinsMichael J Blackman
Jul 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sheetij DuttaDavid E Lanar
Jun 25, 2014·Proceedings of the National Academy of Sciences of the United States of America·Prakash SrinivasanLouis H Miller
Jan 26, 2016·Journal of Molecular Recognition : JMR·Bankala KrishnarjunaRaymond S Norton
Jun 11, 2011·Trends in Parasitology·Jessica S TylerJohn C Boothroyd
May 15, 2010·Trends in Parasitology·Gareth D Weedall, David J Conway
May 16, 2009·Experimental Parasitology·Belinda J MorahanKarena L Waller
Nov 18, 2008·Journal of Cellular and Molecular Medicine·M E PatarroyoM A Patarroyo
Sep 27, 2006·Protein Science : a Publication of the Protein Society·Pernille Haste AndersenOle Lund
Nov 22, 2012·Protein Science : a Publication of the Protein Society·Michelle L TonkinMartin J Boulanger
Apr 6, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Akram Abouie MehriziSedigheh Zakeri
Dec 4, 2012·The Journal of Infectious Diseases·Amed OuattaraChristopher V Plowe
Aug 15, 2014·Frontiers in Immunology·Alyssa E Barry, Alicia Arnott
Aug 31, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P GrynbergE Martins Braga

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.