The MotA transcription factor from bacteriophage T4 contains a novel DNA-binding domain: the 'double wing' motif

Molecular Microbiology
Ning LiStephen W White

Abstract

MotA is a transcription factor from bacteriophage T4 that helps adapt the host Escherichia coli transcription apparatus to T4 middle promoters. We have determined the crystal structure of the C-terminal DNA-binding domain of MotA (MotCF) to 1.6 A resolution using multiwavelength, anomalous diffraction methods. The structure reveals a novel DNA-binding alpha/beta motif that contains an exposed beta-sheet surface that mediates interactions with the DNA. Independent biochemical experiments have shown that MotCF binds to one surface of a single turn of DNA through interactions in adjacent major and minor grooves. We present a model of the interaction in which beta-ribbons at opposite corners of the six-stranded beta-sheet penetrate the DNA grooves, and call the motif a 'double wing' to emphasize similarities to the 'winged-helix' motif. The model is consistent with data on how MotA functions at middle promoters, and provides an explanation for why MotA can form non-specific multimers on DNA.

References

Jan 1, 1992·Annual Review of Biochemistry·C O Pabo, R T Sauer
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Sep 1, 1974·Journal of Bacteriology·F C NeidhardtD F Smith
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·M OuhammouchE N Brody
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M S FinninS W White
Feb 1, 1994·American Journal of Respiratory Cell and Molecular Biology·A R SousaR N Poston
Oct 7, 1993·Nature·Y KimP B Sigler
Jan 1, 1993·Journal of Bacteriology·G OrsiniE N Brody
Nov 1, 1995·Trends in Biochemical Sciences·L Holm, C Sander
Nov 15, 1996·Journal of Molecular Biology·E SeverinovaS A Darst
Apr 15, 1997·The EMBO Journal·M S FinninK N Kreuzer
Jun 24, 1998·Journal of Molecular Biology·E SeverinovaS A Darst
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·R M Esnouf
Feb 19, 2000·Current Opinion in Structural Biology·K S Gajiwala, S K Burley
Jan 1, 1997·Methods in Enzymology·E A Merritt, D J Bacon
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Mar 12, 2003·Journal of Molecular Biology·Lidija TruncaiteRimas Nivinskas
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Robert A MoxleySuchareeta Mitra
May 10, 2012·Nucleic Acids Research·Rasa SukackaiteVirginijus Siksnys
May 24, 2006·Virology Journal·James M NolanJim D Karam
Oct 30, 2010·Virology Journal·Deborah M Hinton
May 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Aaron C MasonBrandt F Eichman
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Sep 28, 2005·Journal of Molecular Biology·Brian D GregoryAnn Hochschild
May 25, 2010·Journal of Molecular Biology·Margaret A JohnsonKurt Wüthrich
Apr 6, 2004·Structure·E Allen SickmierStephen W White
Jul 28, 2016·Nucleic Acids Research·Tamara D JamesDeborah M Hinton
Nov 10, 2006·The Journal of Biological Chemistry·Scott W Nelson, Stephen J Benkovic
Aug 2, 2013·The Journal of Biological Chemistry·Meng-Lun HsiehDeborah M Hinton
Aug 9, 2019·Nucleic Acids Research·Jing ShiYu Feng
May 20, 2003·The Journal of Biological Chemistry·Masood Kamali-Moghaddam, E Peter Geiduschek
Feb 6, 2017·Transcription·Deborah M Hinton
Jun 6, 2017·Protein Science : a Publication of the Protein Society·Robert G HammondMargaret A Johnson
Sep 20, 2007·The Journal of Biological Chemistry·Iain D KerrStephen W White

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.