The mouse homeobox gene, Gbx2: genomic organization and expression in pluripotent cells in vitro and in vivo

Genomics
G ChapmanP D Rathjen

Abstract

The Gbx2 homeodomain is widely conserved in metazoans. We investigated the mouse Gbx2 locus by isolation and characterization of genomic clones and by physical localization to the genome. The Gbx2 gene contained a single intron that separated the proposed functional protein domains. This organization was conserved with human GBX2. Physical localization of Gbx2 to Chromosome 1C5-E1 indicated that the genomic relationship between the linked Gbx2 and En1 genes differs between mouse and human, making it unlikely to be of functional significance. We also extended the known expression pattern of Gbx2 beyond the gastrulation stage embryo and the developing CNS to pluripotent cells in vitro and in vivo. Gbx2 expression was demonstrated in undifferentiated embryonic stem cells but was downregulated in differentiated cell populations. In the embryo, Gbx2 expression was detected before primitive streak formation, in the inner cell mass of the preimplantation embryo. Gbx2 is therefore a candidate control gene for cell pluripotency and differentiation in the embryo.

References

Jan 24, 1992·Cell·W McGinnis, R Krumlauf
Apr 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Holland
Jan 1, 1992·Cytogenetics and Cell Genetics·Y MatsudaV M Chapman
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M M Shen, P Leder
Oct 1, 1991·Trends in Genetics : TIG·E BoncinelliF Mavilio
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M T MurthaF H Ruddle
Oct 1, 1991·Trends in Genetics : TIG·H R Schöler
May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T BoehmT H Rabbitts
Mar 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·I Kemler, W Schaffner
Jul 3, 1989·FEBS Letters·A Fainsod, Y Greunbaum
Jul 28, 1989·Biochimica Et Biophysica Acta·M P ScottG W Hartzell
Jan 1, 1989·Cytogenetics and Cell Genetics·G C WebbI G Young
Jul 20, 1987·Journal of Molecular Biology·M Gardiner-Garden, M Frommer
Jan 1, 1973·Chromosoma·M N Nesbitt, U Francke
Jan 22, 1982·Nucleic Acids Research·S M Mount
May 15, 1995·FEBS Letters·G Chapman, P D Rathjen
Feb 24, 1995·The Journal of Biological Chemistry·P Q ThomasP D Rathjen
Oct 11, 1993·Nucleic Acids Research·R de JongF Meijlink
Apr 21, 1994·Nature·C W RobertsS J Korsmeyer
Jul 29, 1994·Cell·W J GehringK Wüthrich
May 1, 1993·Trends in Genetics : TIG·B Rosen, R S Beddington
Dec 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P BouilletP Chambon

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Citations

Oct 2, 2002·FEBS Letters·Rachel Eiges, Nissim Benvenisty
May 15, 1995·FEBS Letters·G Chapman, P D Rathjen
Oct 20, 2004·Biotechnology and Bioengineering·Raj R RaoSteven L Stice
Sep 10, 2003·Mechanisms of Development·Muriel RhinnMichael Brand
Oct 27, 2004·Developmental Biology·Ludovic VallierRoger A Pedersen
Mar 25, 2005·Physiological Reviews·Anna M Wobus, Kenneth R Boheler
Nov 21, 2007·Cell and Tissue Research·Satoshi YamanakaKenneth R Boheler
Sep 4, 2004·Stem Cells and Development·Jonathan S DraperPeter W Andrews
Dec 30, 2004·Development·Thomas P Zwaka, James A Thomson
Jun 12, 2003·Gene Expression Patterns : GEP·Samuel T WatersMark Lewandoski

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