The mouse mammary tumor cell line, MMT060562, produces prostaglandin E2 and leukemia inhibitory factor and supports osteoclast formation in vitro via a stromal cell-dependent pathway

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
T AkatsuN Nagata

Abstract

Osteoclastic bone resorption increases at the site of bone metastasis, but little is known about how tumor cells induce osteoclast (OC) recruitment in the bone marrow microenvironment. To clarify this point, we examined the effects of various mouse tumor cells on OC recruitment using cocultures of tumor cells and mouse marrow cells. The mouse mammary tumor cell lines, MMT060562 (MMT), BALB/c-MC, Jyg-MC(A), or other nonmammary tumor cell lines, LLC and B16, were cocultured with mouse marrow cells, and OC recruitment from marrow cells was determined by counting the number of tartrate-resistant acid phosphatase-positive multinucleated cells (TRAP(+) MNCs) formed. Of the tumor cells examined, MMT and BALB/c-MC stimulated OC formation, but other tumor cells did not. OC formation with MMT was dependent on the number of MMTs inoculated, and only ten cells per well were sufficient to induce OC development. OCs appeared on day 4, and the number reached a maximum on days 5-8 and decreased thereafter. TRAP(+) MNCs induced by MMT satisfied the major criteria of OCs, such as the presence of calcitonin receptors and the ability to resorb calcified tissues. The majority of OCs were formed adjacent to the stromal cells, which were positive for...Continue Reading

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Citations

Aug 26, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T AkatsuN Nagata
Nov 6, 2003·Clinical Orthopaedics and Related Research·Manali BendreLarry J Suva
Mar 5, 2004·International Journal of Cancer. Journal International Du Cancer·Hayley MorganPeter A Hill
Jul 5, 2001·British Journal of Cancer·N C HuntN A Athanasou
May 15, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Katsuhiro OnoNobuo Kugai
Aug 15, 2002·American Journal of Physiology. Cell Physiology·Ashraf A RagabEdward M Greenfield
Jun 17, 2000·Endocrine Reviews·C J Auernhammer, S Melmed
Mar 27, 2001·International Journal of Cancer. Journal International Du Cancer·A TumberP A Hill
Aug 2, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·G D Roodman
Sep 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bénédicte BrounaisFrédéric Blanchard
Dec 2, 2020·Pharmacology & Therapeutics·Cen ZhangZhaohui Feng
Oct 12, 2000·Cytokine·D Heymann, A V Rousselle

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