PMID: 8943048Nov 26, 1996Paper

The mouse mammary tumor virus env gene is the source of a CD8+ T-cell-stimulating peptide presented by a major histocompatibility complex class I molecule in a murine thymoma

Proceedings of the National Academy of Sciences of the United States of America
S MalarkannanN Shastri

Abstract

CD8+ cytotoxic T cells recognize their targets by the presence of unique peptide bound to a major histocompatibility complex (MHC) class I molecules on the cell surface. The MHC molecules normally display thousands of distinct peptides, making it difficult to identify individual antigenic peptides, their protein precursors, and their relative importance in the T-cell response. Here we used the EL-4 tumor-specific lacZ-inducible KZ30.6 T cell as a probe for detecting the peptide/MHC ligand that was generated in cells transfected with an EL-4 cDNA library. These expression screens allowed identification of a mouse mammary tumor virus (MMTV) transcript as the source of the antigenic peptide presented by the Kb MHC molecule. The antigenic activity was encoded within the MMTV env gene and was defined by the octapeptide ANYDFICV (AFV8). Synthetic AFV8 stimulated KZ30.6 T cells at picomolar concentrations and coeluted with one of two active peptides in HPLC-fractionated extracts of EL-4 cells. The AFV8/Kb complex was also recognized by two other EL-4-specific T cells. The results illustrate a novel strategy for identifying T-cell-stimulating antigens and suggest that the MMTV env gene and its naturally processed AFV8 peptide product c...Continue Reading

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Citations

Nov 22, 1997·Current Opinion in Immunology·E Simpson, D Roopenian
Aug 13, 2015·Cancer Immunology, Immunotherapy : CII·Stefano UgelAndrea Facciabene
Oct 29, 2004·PLoS Biology·Susan R SchwabNilabh Shastri
Mar 31, 2016·Nature Reviews. Immunology·George Kassiotis, Jonathan P Stoye
Feb 11, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·George Kassiotis
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·T van HallR Offringa
Dec 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vincenzo BrontePaola Zanovello
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·H E StefanskiK A Hogquist
Oct 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S AkileshD C Roopenian
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Reinhold SchirmbeckJörg Reimann

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