The mouse relaxin-like factor gene and its promoter are located within the 3' region of the JAK3 genomic sequence

FEBS Letters
P KoskimiesR Ivell

Abstract

Isolation and sequencing of a genomic clone encoding the mouse gene for the relaxin-like factor (RLF), which is endogenously expressed to a high level exclusively in Leydig cells, indicated that similar sequences were also present at the 3' end of the mouse JAK3 gene, a gene expressed predominantly in lymphoid tissues. More extensive Southern blot, polymerase chain reaction and sequencing analyses showed that the published mouse sequence for exon 23 of the JAK3 gene in fact comprises two exons, 23A and 23B, separated by an additional novel intron of 2.2 kb, and that within this intron the promoter and exon 1 of the mouse RLF gene are encoded. The two overlapping transcripts appear to use different polyadenylation signals in the common 3' untranslated region of exon 23B. Transient transfection of different RLF promoter reporter constructs into Leydig, Sertoli, granulosa and kidney cell lines indicate that as little as 0.7 kb of the region upstream of exon 1 of the RLF gene, and within the novel intron 22 of the JAK3 gene, is sufficient to account for cell-specific expression of the RLF gene. This promoter region is specifically hypomethylated in Leydig cells compared to non-expressing tissues.

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Citations

May 20, 1999·Molecular Reproduction and Development·S Hombach-KlonischT Klonisch
Nov 5, 1999·Molecular Reproduction and Development·A N SpiessR Ivell
Feb 25, 2000·Molecular and Cellular Endocrinology·S Hombach-KlonischT Klonisch
Feb 1, 2003·Experimental Gerontology·H J PaustA K Mukhopadhyay
Mar 31, 2009·Human Reproduction Update·Richard Ivell, Ravinder Anand-Ivell
Aug 24, 2002·Biology of Reproduction·Richard Ivell, Ross A D Bathgate
Aug 12, 2015·Scientific Reports·Imteyaz QamarArul Narayanasamy
Jun 16, 2005·Annals of the New York Academy of Sciences·Richard IvellRavinder Anand-Ivell
May 2, 2013·Molecular and Cellular Endocrinology·Richard IvellRavinder Anand-Ivell
Jul 12, 2005·Molecular and Cellular Endocrinology·Helen SadeghianRichard Ivell
Jan 10, 2018·Frontiers in Physiology·Yanzhenzi DaiRavinder Anand-Ivell
Mar 7, 2001·Molecular Biology and Evolution·T KlonischS Hombach-Klonisch
Sep 12, 2018·Human Reproduction Update·Richard Ivell, Ravinder Anand-Ivell
Aug 18, 2020·General and Comparative Endocrinology·Richard IvellRavinder Anand-Ivell
Aug 7, 2007·Biochemistry·Erika E Büllesbach, Christian Schwabe

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