Abstract
The introduction of alternative systems in vivo is very important for cancer patients who are treated with gonadotoxic treatment. In this study, we examine the progression of the spermatogenesis process after human spermatogonial stem cell (SSCs) transplantation in vivo and in tissue culture conditions. Human SSCs were obtained from a Testicular Sperm Extractions (TESE) sample, and characterization of these cells was confirmed by detecting the promyelocytic leukemia zinc finger (PLZF) protein. These cells, after being labeled with Di-alkyl Indocarbocyanine (DiI), were transplanted to adult azoospermia mouse testes treated with Busulfan 40mg/kg. The host testicular tissue culture was then considered a test group and in vivo transplant a control group. After 8 weeks, immunohistochemical, morphometric and molecular studies were performed. The results of morphometric studies indicated that the mean number of spermatogonia, spermatocytes, and spermatids in the test groups was significantly lower than in the control group (P<0.05) and most of the cells responded positively to DiI tracing. Immunohistochemical study in both groups revealed expression of PLZF, Synaptonemal complex protein 3 (SCP3) and Acrosin Binding Protein (ACRBP) pro...Continue Reading
References
May 30, 1996·Nature·D E ClouthierR L Brinster
Jan 23, 1999·Biology of Reproduction·T OgawaR L Brinster
Oct 21, 1999·Biology of Reproduction·I DobrinskiR L Brinster
Jun 17, 2000·Zygote : the Biology of Gametes and Early Embryos·M M ReisG D Palermo
Oct 3, 2000·Molecular Reproduction and Development·I DobrinskiR L Brinster
Apr 25, 2001·Biology of Reproduction·M NaganoR L Brinster
Aug 16, 2002·Nature·Ali HonaramoozStefan Schlatt
Dec 13, 2002·Fertility and Sterility·Makoto NaganoRalph L Brinster
May 30, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Richard M SharpeJane S Fisher
Jun 23, 2007·Stem Cells·Brian P HermannKyle E Orwig
Nov 6, 2008·Cell Stem Cell·Mito Kanatsu-ShinoharaTakashi Shinohara
Nov 3, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Brian P HermannKyle E Orwig
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xin WuRalph L Brinster
Apr 16, 2010·Biology of Reproduction·Ayako GohbaraTakehiko Ogawa
Feb 26, 2011·Human Reproduction·Fariborz IzadyarAlan Copperman
Mar 25, 2011·Nature·Takuya SatoTakehiko Ogawa
Aug 3, 2011·Andrologia·T MirzapourS H Rafieian
Sep 15, 2011·Nature Communications·Takuya SatoTakehiko Ogawa
Feb 24, 2012·Molecular Human Reproduction·Steve TardifGail A Cornwall
May 18, 2012·Biology of Reproduction·Khaled ZohniMakoto Nagano
Sep 21, 2012·Methods in Molecular Biology·Tetsuhiro YokonishiTakehiko Ogawa
Dec 19, 2012·Spermatogenesis·Hye-Won Song, Miles F Wilkinson
Oct 5, 2013·Nature Protocols·Takuya SatoTakehiko Ogawa
Jul 1, 2014·Reproduction in Domestic Animals = Zuchthygiene·N R HanS T Lee
Apr 25, 2016·Current Urology Reports·Guillermo GaldonHooman Sadri-Ardekani
May 4, 2017·Animal Reproduction Science·Won-Young LeeHyuk Song
Jun 24, 2017·Current Stem Cell Research & Therapy·Azar ShamsMorteza Koruji
Aug 31, 2017·Development·Dirk G de Rooij
Oct 17, 2017·Oncotarget·Fahar IbtishamGuanghui Li