The movement protein of cucumber mosaic virus traffics into sieve elements in minor veins of nicotiana clevelandii

The Plant Cell
L M BlackmanKarl J Oparka

Abstract

The location of the 3a movement protein (MP) of cucumber mosaic virus (CMV) was studied by quantitative immunogold labeling of the wild-type 3a MP in leaves of Nicotiana clevelandii infected by CMV as well as by using a 3a-green fluorescent protein (GFP) fusion expressed from a potato virus X (PVX) vector. Whether expressed from CMV or PVX, the 3a MP targeted plasmodesmata and accumulated in the central cavity of the pore. Within minor veins, the most extensively labeled plasmodesmata were those connecting sieve elements and companion cells. In addition to targeting plasmodesmata, the 3a MP accumulated in the parietal layer of mature sieve elements. Confocal imaging of cells expressing the 3a-GFP fusion protein showed that the 3a MP assembled into elaborate fibrillar formations in the sieve element parietal layer. The ability of 3a-GFP, expressed from PVX rather than CMV, to enter sieve elements demonstrates that neither the CMV RNA nor the CMV coat protein is required for trafficking of the 3a MP into sieve elements. CMV virions were not detected in plasmodesmata from CMV-infected tissue, although large CMV aggregates were often found in the parietal layer of sieve elements and were usually surrounded by 3a MP. These data sugg...Continue Reading

Citations

Mar 22, 2002·Trends in Plant Science·Aart J E van BelMichael Knoblauch
Aug 27, 1999·Trends in Plant Science·G A Thompson, A Schulz
Jun 22, 1999·Current Opinion in Plant Biology·W J Lucas, S Wolf
Jan 1, 2003·Molecular Plant Pathology·Massimo TurinaHerman B Scholthof
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·N H ChengR S Nelson
Jul 12, 2008·Molecular Plant-microbe Interactions : MPMI·Tefera MekuriaJeanmarie Verchot-Lubicz
Aug 15, 2008·Molecular Plant-microbe Interactions : MPMI·Rebecca LyonsKostya Kanyuka
Feb 13, 2003·Molecular Plant-microbe Interactions : MPMI·Minna-Liisa Rajamäki, Jari P T Valkonen
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Karl J. Oparka, Simon Santa Cruz
Jan 15, 2011·Virology Journal·Giovanni Chaves-BedoyaLaura Silva-Rosales
Jan 30, 2007·PLoS Pathogens·Fernando EscriuFernando García-Arenal
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mohammed BendahmaneRoger N Beachy
Feb 2, 1999·Molecular Plant-microbe Interactions : MPMI·L Simón-Buela, F García-Arenal
Sep 12, 2001·Molecular Plant-microbe Interactions : MPMI·J A Sánchez-Navarro, J F Bol
Sep 6, 2011·Plant Science : an International Journal of Experimental Plant Biology·Aart J E van BelJohn W Patrick
Sep 10, 2008·Journal of Microscopy·D Y T LiuR L Overall
Jun 15, 2007·The Plant Journal : for Cell and Molecular Biology·Gadi PelegShmuel Wolf
Jul 19, 2005·Trends in Plant Science·Herman B Scholthof
May 25, 2002·The Journal of General Virology·M S SilvaJ W M van Lent
Mar 21, 2001·The Journal of General Virology·T CantoP Palukaitis
Dec 4, 2014·Molecular Plant Pathology·Cèlia Guiu-AragonésAna Montserrat Martín-Hernández
Aug 25, 1999·Current Opinion in Plant Biology·S G Lazarowitz
Jan 30, 2008·Current Protocols in Cell Biology·C HawesI Moore
Dec 25, 2019·Journal of Experimental Botany·Dawei Yan, Yao Liu

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.