The MRTF-A/miR-155/SOX1 pathway mediates gastric cancer migration and invasion

Cancer Cell International
Libin YinLei Wang

Abstract

Gastric cancer (GC) is the leading cause of death worldwide and is closely related to metastasis. MRTF-A is one of the most well-characterized genetic markers in cancer. However, the mechanism whereby MRTF-A mediate gastric cancer (GC) tumorigenesis is not fully clear. Increasing evidence has confirmed that miRNA dysregulation is involved in MRTF-A-mediated tumorigenesis, supporting their potential as therapeutic targets for cancer. Although miR-155 has been reported as an upregulated miRNA, the interplay between miR-155 and MRTF-A-mediated gastric cancer progression remain largely elusive. Real-time PCR was performed to determine miR-155 expression after transfected with MRTF-A encoding plasmids and siRNA. Potential target genes were identified by Western blot and luciferase reporter assay. Chip assay was proved that MRTF-A binds in the promoter region of miR-155. Transwell assay and Scratch-healing migration assay was used to investigate the role of MRTF-A and SOX1 in gastric cancer cell migration and invasion. MRTF-A can interact with the miR-155 promoter to promote histone acetylation and RNA polymerase II recruitment via the Wnt-β-catenin pathway. miR-155 promotes gastric cancer cell migration by suppressing SOX1 expressio...Continue Reading

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Citations

Jan 17, 2021·International Journal of Molecular Sciences·Radu PirlogIoana Berindan-Neagoe
Jan 1, 2021·Bioorganic Chemistry·Xin-Hui ZhangHong-Min Liu
Mar 5, 2021·International Journal of Biological Macromolecules·Asal Jalal AbadiMichael R Hamblin

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Methods Mentioned

BETA
histone acetylation
PCR
immunoprecipitation
ChIP
transfection
Assay
nuclear translocation
acetylation
xenograft
xenografts

Software Mentioned

Targetscan
BLAST
Primer
SPSS
LabWorks Image Acquisition and Analysis

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