The MSX1 homeobox transcription factor is a downstream target of PHOX2B and activates the Delta-Notch pathway in neuroblastoma.

Experimental Cell Research
Ingrid RevetDirk Geerts

Abstract

Neuroblastoma is an embryonal tumour of the peripheral sympathetic nervous system (SNS). One of the master regulator genes for peripheral SNS differentiation, the homeobox transcription factor PHOX2B, is mutated in familiar and sporadic neuroblastomas. Here we report that inducible expression of PHOX2B in the neuroblastoma cell line SJNB-8 down-regulates MSX1, a homeobox gene important for embryonic neural crest development. Inducible expression of MSX1 in SJNB-8 caused inhibition of both cell proliferation and colony formation in soft agar. Affymetrix micro-array and Northern blot analysis demonstrated that MSX1 strongly up-regulated the Delta-Notch pathway genes DLK1, NOTCH3, and HEY1. In addition, the proneural gene NEUROD1 was down-regulated. Western blot analysis showed that MSX1 induction caused cleavage of the NOTCH3 protein to its activated form, further confirming activation of the Delta-Notch pathway. These experiments describe for the first time regulation of the Delta-Notch pathway by MSX1, and connect these genes to the PHOX2B oncogene, indicative of a role in neuroblastoma biology. Affymetrix micro-array analysis of a neuroblastic tumour series consisting of neuroblastomas and the more benign ganglioneuromas showe...Continue Reading

References

Jan 1, 1989·Cytogenetics and Cell Genetics·M K RitkeA Tereba
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M SchwabJ M Bishop
Jun 1, 1980·European Journal of Biochemistry·C Auffray, F Rougeon
Oct 1, 1995·Trends in Genetics : TIG·D Davidson
Feb 6, 1999·International Journal of Cancer. Journal International Du Cancer·M B DiccianniA L Yu
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M LiuD H Lowenstein
Mar 4, 2000·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·V V Joshi
Mar 11, 2000·The Journal of Clinical Investigation·A JoutelE Tournier-Lasserve
Aug 31, 2000·Biochemical and Biophysical Research Communications·M M Maier, M Gessler
Nov 30, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T A JonesD Sheer
Feb 13, 2001·Cell·S J OdelbergM T Keating
Mar 13, 2001·Biochemical and Biophysical Research Communications·J ParkJ H Lee
Mar 30, 2001·Journal of Neurochemistry·J H ChoH Suh-Kim
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·C Blin-WakkachA Berdal
Dec 1, 2001·Journal of Clinical Pathology·N Bown
Jul 3, 2002·Nature Reviews. Neuroscience·Nicolas BertrandFrançois Guillemot
Jan 28, 2003·Journal of Cellular Physiology·Tatsuya IsoYasuo Hamamori
Jan 31, 2003·Genes, Chromosomes & Cancer·Jan J MolenaarHuib N Caron
Mar 4, 2003·Nature Reviews. Cancer·Garrett M Brodeur
Apr 3, 2003·International Journal of Cancer. Journal International Du Cancer·Vera A E Van LimptRogier Versteeg
Jul 23, 2003·Development·Antoine BachBenoît Robert
Aug 7, 2003·The Lancet Oncology·Manfred SchwabFrank Berthold
Oct 3, 2003·Nature Reviews. Neuroscience·Laura S Gammill, Marianne Bronner-Fraser
Feb 20, 2004·Development·Ying LiuJane E Johnson
Mar 17, 2004·American Journal of Human Genetics·Delphine TrochetJeanne Amiel
Aug 4, 2004·Seminars in Cancer Biology·Emil M HanssonGavin Chapman
Sep 1, 2004·American Journal of Human Genetics·Yael P MosseJohn M Maris
Sep 11, 2004·Development·Nicole M Le DouarinElisabeth Dupin
Sep 15, 2004·Developmental Cell·Daniel Meulemans, Marianne Bronner-Fraser
Nov 2, 2004·Oncogene·Vera van LimptRogier Versteeg
Jan 18, 2005·Experimental Cell Research·Victoriano BaladrónJorge Laborda

❮ Previous
Next ❯

Citations

Oct 20, 2009·BMC Cancer·Stefan NagelRoderick Af Macleod
Feb 27, 2013·International Journal of Oncology·Dana-Lynn T KoomoaAndré S Bachmann
Oct 22, 2008·Current Oncology Reports·Patrick A Zweidler-McKay
Jan 29, 2013·Oral Oncology·Caroline AbadieMartine Bonnaure-Mallet
Jan 4, 2012·International Journal of Cancer. Journal International Du Cancer·Joanna E GaweckaJoe W Ramos
Jul 1, 2014·Cancer Medicine·Michael WeichhausLinda Connelly
Dec 5, 2009·International Journal of Cancer. Journal International Du Cancer·Dirk GeertsAndré S Bachmann
Mar 30, 2012·The Biochemical Journal·Mitchell E MenezesRajeev S Samant
Jan 14, 2017·Journal of Pediatric Hematology/oncology·Alper OzcanEkrem Unal
Feb 2, 2017·Molecular Cancer·Stephanie Tsang Mui ChungLinda Connelly
Aug 19, 2010·Neuro-oncology·Giulia Ferrari-ToninelliMaurizio Memo
Dec 10, 2017·Cell and Tissue Research·Julie A TomolonisJason M Shohet
May 12, 2018·PLoS Biology·Marie MénardServane Tauszig-Delamasure
Jan 13, 2018·Cell Death and Differentiation·Jessy HasnaPhilippe Kischel
May 16, 2009·Clinical Chemistry·Bertil Kågedal
Aug 25, 2019·Clinical Genetics·Tiziana Bachetti, Isabella Ceccherini
Jan 14, 2020·Cancer Metastasis Reviews·Gian Paolo Tonini, Mario Capasso
Jul 9, 2017·Scientific Reports·Marta NetoFernando Casares
Jul 8, 2020·International Journal of Molecular Sciences·Simon EppichHelene Hildegard Heidegger
Feb 27, 2013·International Journal of Oncology·Lisette P YcoAndré S Bachmann
May 20, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Liu-Ting ChenHong-Bing Shu
Aug 26, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hsiu-Hao ChangYung-Feng Liao
Nov 7, 2020·Medicinal Research Reviews·Atif ZafarRuiwen Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Fate Specification

Cell fate specification is determined by complex signal transduction pathways including Notch and the Sonic hedgehog signaling pathway. Here is the latest research on regulation of cell fate determination.