The mu opioid receptor modulates neurotransmission in the rat spinal ventral horn

Anesthesia and Analgesia
Hiroyuki HondaTatsuro Kohno

Abstract

Opioids inhibit excitatory neurotransmission and produce antinociception through μ opioid receptors (MORs). Although MORs are expressed in the spinal ventral horn, their functions and effects are largely unknown. Therefore, we examined the neuromodulatory effects of μ opioids in spinal lamina IX neurons at the cellular level. The effects of the selective μ agonist [D-Ala(2),-N-Me-Phe(4), Gly(5)-ol]enkephalin (DAMGO) on synaptic transmission were examined in spinal lamina IX neurons of neonatal rats using the whole-cell patch-clamp technique. DAMGO produced outward currents in 56% of the lamina IX neurons recorded, with a 50% effective concentration of 0.1 μM. Analysis of the current-voltage relationship revealed a reversal potential of approximately -86 mV. These currents were not blocked by tetrodotoxin but were inhibited by Ba(2+) or a selective μ antagonist. Moreover, the currents were suppressed by the addition of Cs(+) and tetraethylammonium or guanosine 5'-[β-thio]diphosphate trilithium salt to the pipette solution. In addition, DAMGO decreased the frequency of spontaneous excitatory and inhibitory postsynaptic currents, and these effects were unaltered by treatment with tetrodotoxin. Our results suggest that DAMGO hyperp...Continue Reading

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Citations

Oct 16, 2013·Peptides·Richard J Bodnar
Oct 30, 2015·The Journal of Physiology·Masayuki OhashiTatsuro Kohno
Aug 30, 2017·Frontiers in Neural Circuits·Zhen-Yu WuYun-Qing Li
Oct 15, 2020·Physiology·Barbara PalkovicAstrid G Stucke

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