Apr 1, 2020

A trimeric autotransporter enhances biofilm cohesiveness in Yersinia pseudotuberculosis but not in Yersinia pestis

BioRxiv : the Preprint Server for Biology
Joshua T CalderDavid L Erickson


Cohesion of biofilms made by Yersinia pestis and Yersinia pseudotuberculosis (Yptb) has been attributed solely to an extracellular polysaccharide matrix encoded by the hms genes (Hms-ECM). However, mutations in the Yptb BarA/UvrY/CsrB regulatory cascade enhance biofilm stability without dramatically increasing Hms-ECM production. We found that treatment with proteinase K enzyme effectively destabilized Yptb csrB mutant biofilms, suggesting that cell-cell interactions might be mediated by protein adhesins or extracellular matrix proteins. We identified an uncharacterized trimeric autotransporter lipoprotein (YPTB2394), repressed by csrB, which has been referred to as YadE. Biofilms made by a DyadE mutant strain were extremely sensitive to mechanical disruption. Overexpression of yadE in wild-type Yptb increased biofilm cohesion, similar to biofilms made by csrB or uvrY mutants. We found that the Rcs signaling cascade, which represses Hms-ECM production, activated expression of yadE. The yadE gene appears to be functional in Yptb but is a pseudogene in modern Y. pestis strains. Expression of functional yadE in Y. pestis KIM6+ altered the production of Hms-ECM and weakened biofilms made by these bacteria. This suggests that althou...Continue Reading

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Mentioned in this Paper

Gene Polymorphism
Genome-Wide Association Study
Ion Homeostasis
Dysequilibrium Syndrome
Genetic Research
Liver Diseases
Heterogeneous Nuclear RNA
Mice, Knockout

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