The multi-KH domain protein of Saccharomyces cerevisiae Scp160p contributes to the regulation of telomeric silencing

The Journal of Biological Chemistry
Francesc-Xavier MarsellachFernando Azorín

Abstract

Multi-KH domain proteins are highly evolutionarily conserved proteins that associate to polyribosomes and participate in RNA metabolism. Recent evidence indicates that multi-KH domain proteins also contribute to the structural organization of heterochromatin both in mammals and Drosophila. Here, we show that the multi-KH domain protein of Saccharomyces cerevisiae, Scp160p, contributes to silencing at telomeres and at the mating-type locus, but not to ribosomal silencing. The contribution of Scp160p to silencing is independent of its binding to the ribosome as deletion of the last two KH domains, which mediate ribosomal binding, has no effect on silencing. Disruption of SCP160 increases cell ploidy but this effect is also independent of the contribution of Scp160p to telomeric silencing as strong relief of silencing is observed in Deltascp160 cells with normal ploidy and, vice versa, Deltascp160 cells with highly increased ploidy show no significant silencing defects. The TPE phenotype of Deltascp160 cells associates to a decreased Sir3p deposition at telomeres and, in good agreement, silencing is rescued by SIR3 overexpression and in a Deltarif1Deltarif2 mutant. Scp160p shows a distinct perinuclear localization that is independ...Continue Reading

References

Jan 2, 1992·Gene·T W ChristiansonP Hieter
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·S E RundlettM Grunstein
Jan 1, 1997·Genes & Development·S Strahl-BolsingerM Grunstein
Jan 15, 1997·Genes & Development·J S Smith, J D Boeke
Jun 1, 1997·Current Opinion in Cell Biology·K Nasmyth, R P Jansen
Mar 10, 2000·Nucleic Acids Research·B D Lang, J L Fridovich-Keil
Jun 22, 2000·Yeast·V V Kushnirov
Feb 13, 2001·Nucleic Acids Research·N V Grishin
Nov 6, 2002·Molecular and Cellular Biology·Erik D AndrulisRolf Sternglanz
Mar 26, 2003·Nucleic Acids Research·Ai-Min LiJudith L Fridovich-Keil
Apr 5, 2003·Annual Review of Biochemistry·Laura N RuscheJasper Rine
Oct 8, 2003·Nucleic Acids Research·Bryce A MendelsohnJudith L Fridovich-Keil
Mar 24, 2004·Current Opinion in Cell Biology·Miguel López de Heredia, Ralf-Peter Jansen
Apr 28, 2004·The International Journal of Biochemistry & Cell Biology·Tillman VollbrandtCharli Kruse
Sep 10, 2004·Nucleic Acids Research·Ai-min LiJudith L Fridovich-Keil
Feb 23, 2005·Current Biology : CB·Qiaoqiao WangGordon G Carmichael

❮ Previous
Next ❯

Citations

Nov 14, 2008·Molecular Biology of the Cell·Muhammad Attiq RehmanKrassimir Yankulov
Jan 22, 2014·Nucleic Acids Research·Wolf D HirschmannRalf-Peter Jansen
Nov 3, 2010·Biochimica Et Biophysica Acta·Marta BatlleFernando Azorín
Dec 11, 2007·Journal of Molecular Biology·Oleg Denisenko, Karol Bomsztyk
Apr 16, 2010·Current Opinion in Cell Biology·Justin M O'Sullivan
Jul 20, 2019·The FEBS Journal·Birgit Singer-KrügerRalf-Peter Jansen
Oct 5, 2017·Wiley Interdisciplinary Reviews. RNA·Matthew Hk Cheng, Ralf-Peter Jansen
Jun 3, 2015·Cell Biology International·Ling WeiYang Qin

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.